(25) Briefly, [micro]OCT is conducted with a spectral-domain OCT system with a broad bandwidth supercontinuum light source spanning from 650 to 950 nm, which provides an
axial resolution of less than 1 [micro]m in tissue.
'A diagnostic run takes about five minutes and provides images with an
axial resolution of 10pm and a lateral resolution of about 30pm,' explained Tearney.
Limitations include high phototoxicity, insufficient
axial resolution, a need for specialized sample handling devices, and lack of tools for precise compound administration.
While SD-OCT is typically slower than SS-OCT, it offers superior phase stability and
axial resolution, which is beneficial in Doppler flowmetry or capillary imaging that require high phase stability and fine
axial resolution.
They demonstrated that the
axial resolution was higher in AS-OCT compared to USB and that AS-OCT was superior in visualizing lesions involving the lateral and anterior aspects of the iris.
Fortunately, axial bulk motion noise is rejected by split-spectrum amplitude-decorrelation angiography [9], which trades some of the
axial resolution for decreased noise and higher transverse resolution [10].
Image resolution is determined by lateral and
axial resolution. Lateral resolution is the same as in widefield microscopy, and is given by the numerical aperture (NA) of the detection objective and the wavelength.
The functionality and potential of time-domain (TD-OCT) machines was limited due to slow scanning speeds of 400 A-Scans per second and an
axial resolution of 10[micro]m.
The ability of instrument to distinguish between two structures located very close together along axis of ultrasound beam is called its
axial resolution. The
axial resolution is best when groups of waves emitted have a short wave length.