In activated RASFs, ST2 negatively affected IL-33, IL-1[sz], TNF-a, IL-6, and IL-8. In the PBMC culture study, soluble ST2 showed no effects on these inflammatory cytokines which might be attributed to the fact that there was no IL-33 expression in human PBMCs.[17] Therefore, we thought that the negative effects of soluble ST2 on the above inflammatory cytokines might depend on IL-33 levels and these inflammatory cytokines might be downstream of IL-33.
In the present study, we also demonstrated the role of soluble ST2 on cytokines, including IL-33, IL-1[sz], TNF-a, IL-6, and IL-8. After soluble ST2 treatment, the expressions of both IL-1[sz] and IL-33 were downregulated in resting RASFs, but the expressions of TNF-a, IL-6, and IL-8 were not affected.
(b) Cytokine gene expression in IRIS groups before and after HAART; IL-1[beta], IL-6, IL-8, and TNF-[alpha] expression from low to high; TNF-[alpha] gene expression also increased in non-IRIS group after HAART; ** the gene expressions after HAART were higher than those before treatment.
IL-6 and IL-8 expressions were obviously lower in IRIS group; * before HAART, the gene expressions in IRIS group were lower than those in non-IRIS group.
The concentrations of serum IL-6 and urine IL-6,
IL-8, and TNF in HFRS patients increased with the increasing severity of the disease, and differed significantly in each type.
However IL-15R protein expres-sion was induced while the cells were exposed to IL-15,
IL-8, or a combination of IL-1 b and TNF- a.
Two lung tissue sections were prepared for each subject, one to stain for
IL-8 and IgG, and the other to stain for
IL-8 and Fc[gamma]RIIa.
To investigate the influence of storage conditions on the
IL-8 concentration of the lysate samples, lysate obtained from individual EDTA blood samples was aliquoted into 1.5-mL polypropylene tubes (0.2-mL aliquots) and stored for different periods at room temperature, at 4[degrees]C, or frozen at -20 and -80[degrees]C.
Zachariae et al., "
IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis," The Journal of Immunology, vol.
Ascitic fluid
IL-8 concentrations were greater in SBP patients than in the SA group (P <0.000001; Table 1).
Bland-Altman analyses revealed that agreement was moderate for IL-1[beta], IL-5, IL-6,
IL-8, and MIP-1[beta] and low for IL-4, IL-7, IL-12p70, IL-13, G-CSF, IFN-[gamma], TNF-[alpha], and MCP-1.