The tumor sample fragments are compared to the fragments from non-tumor tissue of the same source by overlaying the
electropherograms. The normal tissue is the red trace; the other traces are the tumor sample.
The capillarywas regenerated and conditioned with methanol, water, hydrochloride acid (0.1 M), water, sodium hydroxide (0.1 M), water and BGS for 1 min, 0.5 min, 2 min, 0.5 min, 2 min, 0.5 min and 2 min respectively as reported [34] prior to each run or if any distortion in the peak shape in
electropherograms during the day is observed.
The analysis of the acceptability based on inherent imprecision of both techniques in the rooster's data and the Wilcoxon's test results stress that the 2 methods cannot be considered to be identical, thus exposing the need for specific reference intervals for the correct interpretation of capillary
electropherograms. As previously discussed, such differences could be related to differences in electrophoretic mobility between certain proteins, depending on which technique is used.
Electropherograms were processed with GeneMapper version 3.7 software (ABI, Carlsbad, CA).
Separation was done and all
electropherograms for different peaks according to immigration time and pick area under curve are presented in figure 6.
As an example, the
electropherograms of spiking for the identification of chlorogenic acid in potato extract are presented in Fig.
Eventually, an image called an
electropherogram is produced, showing the different forms of EPO (also called isoforms) present in a urine sample.
Caption: Figure 1: Typical
electropherogram of the DNA sample extracted from whole human blood after formic acid hydrolysis obtained with 50 mmol/L BTP phosphate buffer at pH 3.25 as the run buffer.
This produces an
electropherogram (Figure 1) in which the 28S and 18S molecules provide highly recognizable peaks, which can be automatically analyzed for features including relative ratios of 28S and 18S peak areas to total RNA detected of all sizes; the height of the 28S peak; the ratio of the 28S to 18S peak sizes; and a number of other metrics.
The number of bands and relative amount of glutenin and gliadin subunits were observed within the fol-lowing ranges of molecular weights: below 40 kDa, 40-80 kDa, and above 80 kDa for glutenins and below 30 kDa, 30-75 kDa, and above 75 kDa for gliadins and expressed as a percentage of total area within each
electropherogram [24-27].