Nakano, "Synthesis of oligonucleotide derivatives containing pyrene labeled glycerol linkers: enhanced excimer fluorescence on binding to a
complementary DNA sequence," Tetrahedron Letters, vol.
In 1978, he went to Stanford University School of Medicine as postdoctoral fellow and developed a new technique that enables the cloning of full-length
complementary DNAs on the basis of their functional expression in a given cell of interest.
cDNA (
complementary DNA): A segment of DNA identical in base sequence to at least part of the coding sequence of a gene, generated in the laboratory from a natural mRNA molecule, cDNA can be produced in large quantities for sequencing and other genetic studies.
DNA chips for examining gene expression involve longer fragments of synthetic or
complementary DNA. Applications of these chips include protein correlation studies, sequencing by hybridization and functional genomic applications.
In a method called expressed sequence tagging (EST), researchers isolate these RNA templates from cells and convert them back into a DNA form (
complementary DNA, or cDNA), which is then pasted into carriers.
Like many jumping genes, the P-element has two short, mirror-image sequences at either end, which are thought to facilitate a splicing reaction with the host's DNA; the host cell papers over the gaps with
complementary DNA code.
She plans to take one of these RNA strands from the tomato spotted wilt virus, make a
complementary DNA, then reverse the direction of a portion of that DNA sequence.
The DNA-modified nanoparticles -- gold in this case -- are positioned on a pre-patterned template made of
complementary DNA. Stacks of structures can be made by introducing a second and then a third DNA-modified particle with DNA that is complementary to the subsequent layers.
In recent research, scientists used a genetic therapy in laboratory mice, in which a virus helped carry
complementary DNA into appropriate cells and restored some GluN2B subunits.
Supreme Court on June 13 ruled that companies cannot patent isolated human genes, but can patent human-engineered DNA molecules, including so-called
complementary DNA or cDNA.
Complementary DNA, or cDNA, can be patented as it does not occur naturally.