Mentioned in

References in periodicals archive

Furthermore, we hope that this report will remind laboratorians of the need to understand the amplification characteristics of the thermostable DNA polymerases that they use for PCR-based assays and to periodically review frequently updated SNP databases for uncommon polymorphisms that could disrupt amplification of any given target sequence.

The Power of Proficiency Testing: Unraveling Single-Nucleotide Polymorphism Interference, With Potential Impact on Clinical Testing of Spinocerebellar Ataxia Type 3

It also inhibits B-family DNA polymerases from eukaryotes [18-21], bacteria [22] and viruses [14; 23-25].

Inhibitory Mechanism Exhibited by Phenol-based Natural Products against DNA Polymerase [alpha] from Psoralea corylifolia by Molecular Docking

KlenTaq 1 (DNA Polymerase Technology) and Taq (New England BioLabs) were used in this study.

The Effect of Single Mismatches on Primer Extension

As an enzyme for PCR, we used the following types of DNA-dependent DNA polymerases: Taq DNA polymerase, Tersus DNA polymerase, Deep Vent DNA polymerase and Phusion DNA polymerase.

Systematic comparison of the capability of modified nucleoside triphosphates to act as a substrate for thermostable DNA polymerases during PCR

Hogrefe, "PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases," Nucleic Acids Research, vol.

Error rate comparison during polymerase chain reaction by DNA polymerase

The concentrations used for each DNA polymerase are specified in the respective figure legends.

Effects of stability of base pairs containing an oxazolone on DNA elongation

However, researchers have been trying to produce such enzyme from various versions of Taq DNA polymerase gene via genetic engineering.

ISOLATION, CHARACTERIZATION OF NOVEL FRAGMENT OF A GENE KLENTAQ WHICH ENCODES THERMUS AQUATICUS DNA POLYMERASE AND ITS THERMOSTABILITY STUDIES IN ESCHERICHIA COLI

Another tenable explanation for the presence of multiple specialised DNA polymerases in vertebrates is that each evolved to accurately bypass a particular type of naturally occurring base damage.

The molecular basis of mutagenesis--40 years of research on genomic integrity

Thus, DNA polymerases might sensitively recognize the [gamma]-amidotriphosphate moiety of the substrates, and the geometrical fluctuation caused by non-cognate pairings, such as Ds-Ds and A-Pa, significantly reduces the interaction between the amino acid residues in the polymerase and the [gamma]-amidotriphosphate moiety, resulting in the loss of the function of the amidodiphosphate part as a leaving group, like the pyrophosphate moiety of triphosphate substrates.

Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma

Cloning and sequencing of lcr1 by Pfu DNA polymerase: To determine whether the sequence variants were created during PCR due to Taq polymerase error, lcr1 was amplified from the parasite genomic DNA by Pfu DNA polymerase (Fermentas Co., Ontario, Canada) which exhibits 3'[right arrow]5' exonuclease (proof reading) activity according to manufacturer's instructions.

Complete conservation of an immunogenic gene (lcr1) in Leishmania infantum and Leishmania chagasi isolated from Iran, Spain and Brazil

Studies will determine the in vivo roles of DNA polymerases and other proteins likely to be involved in UV mutagenesis.

MERIT award winners