This is expected, because caspase-8 is a major initiation in death receptor signaling; active caspase-8 leads to the activation of downstream caspases, including
caspase 3 (Chen and Wang, 2002).
Tylophora alkaloids induced apoptosis in K562 cells with characteristic apoptotic features like nuclear condensation, apoptotic body formation, flipping of membrane phosphatidylserine, activation of
caspase 3 and release of mitochondrial cytochrome c.
The primary antibodies used to detect MLKL (37705S), cleaved
caspase 3 (9661s), and
caspase 3 (9662s) were obtained from Cell Signaling Technology (USA).
Expression and activation of
caspase 3 following status epilepticus in the rat.
Objective: To determine the applicability of urinary
caspase 3 enzyme and TNF-a as biomarkers in children with ureteropelvic junction obstruction (UPJO).
As shown in the Figure 4a, the
Caspase 3 activities were significantly increased in 1.6 mg/l of the PFOS single-treatment, 0.8 mg/l of the PFOS and 1.6 mg/l of the PFOS joint exposure groups compared with that in the control (p < 0.05).
Xie et al., "Quercetin induces apoptosis by activating
caspase 3 and regulating Bcl-2 and cyclooxygenase-2 pathways in human HL-60 cells," Acta Biochimica of Biophysica Sinica, vol.
Sun et al., "Prevention of renal ischemic injury by silencing the expression of renal
caspase 3 and caspase 8," Transplantation, vol.
The following primary antibodies were used: WNV (Envelope protein; Abcam, Cambridge, MA), Iba-1 (Wako Chemicals, Richmond, VA), CD3 and Mac-3 (BD Biosciences, San Jose, CA), Fas (X-20, Santa Cruz Biotechnology, Dallas, TX),
caspase 3 (Asp175, clone 5A1E, Cell Signaling, Danvers, MA), and PYCARD ASC-speck (MyBiosource, San Diego, CA).
MCF-7 cancer cell lines are characterized by resistance chemotherapeutic agents, [17,18] responsive estrogen receptors (ER+), overexpression of Bcl-2, [19,20] and with no expression of
Caspase 3. [21,22] Stimulation of ER could increase P-gp concentration.