The protein marker consisted of
triosephosphate isomerase from rabbit muscle (26.6 kDa), myoglobin from horse heart (17.0 kDa), [alpha]-lactalbumin from bovine milk (14.2 kDa), aprotinin from bovine lung (6.5 kDa), insulin chain B, oxidized, bovine (3.496 kDa), and bradykinin (1.06 kDa).
aureus as described previously, including carbamate kinase (arcC), shikimate dehydrogenase (aroE), glycerol kinase (glp), Guanylate kinase (gmk), phosphate acetyltransferase (pta),
triosephosphate isomerase (tpi), and acetyl coenzyme A acetyltransferase (yqi).
The standard TIM coupled assay [10] contained glyceraldehyde-3-phosphate (0.4 mM), NADH (0.1 mM), [alpha]-glycerophosphate dehydrogenase (0.5 units/ mL), and
triosephosphate isomerase (rabbit muscle TIM) (0.01 units/mL) in 0.1 M triethanolamine hydrochloride buffer, pH 8.0 [10].
Crystal structures of P falciparum glycolytic enzymes aldolase (PfALDO) [11],
triosephosphate isomerase (PfTPI) [12], glyceraldehyde 3-phosphate dehydrogenase (PfGAPDH) [13], lactate dehydrogenase (PfLDH) [14], glucose 6-phosphate isomerase (PfGPI) (Gileadi et al.
Knowles, "Stabilization of a reaction intermediate as a catalytic device: definition of the functional role of the flexible loop in
triosephosphate isomerase," Biochemistry, vol.
farinae allergens, Der f 25 is a
triosephosphate isomerase (TPI) with a molecular weight of 34kDa, showing 75.6% by immunoblotting and 60% by skin prick positive reaction to dust mite allergic patients, respectively.
To our knowledge, only a limited number of enzymes with such catalytic carboxyl group(s) are known so far in other groups of enzymes than hydrolases and transferases, such as
triosephosphate isomerase (catalytic Glu), (201) glucosamine 6-phosphate isomerase (or deaminase) (catalytic Asp), (202) phosphofructokinase (catalytic Asp) 203) etc., where the catalytic Glu/Asp residues are also thought to function as general acid/bases.
The isolates were identified by the rapid ID32A system (bioMerieux, Inc., Durham, North Carolina, United States of America) and polymerase chain reaction amplification of the
triosephosphate isomerase gene.
bieneusi genotypes in the fecal specimens and differentiated them by using PCR and sequence analysis of the small subunit rRNA gene (5),
triosephosphate isomerase gene (6), and ribosomal internal transcribed spacer (4), respectively.
McKenna and Farrell (2010) used DNA sequences from 9 nuclear genes (elongation factor-1[alpha] (EF-1[alpha]), alanyl-tRNA synthetase (AATS), CAD, 6-phosphogluconate dehydrogenase (PGD), sans fille (SNF),
triosephosphate isomerase (TPI), RNA Pol II, 28S, and 18S) to reconstruct the phylogeny of Holometabola with a focus on determining the phylogenetic placement of Strepsiptera.