The concentration of magnesium ions directly impacts the
ribozyme's movements.
The HIV-1 NL43 genomic sequence was analyzed for the presence of potential hammerhead
ribozyme target sites.
Inhibition of HPV-16 E6/E7 immortalization of normal keratinocytes by hairpin
ribozymes. Proc Natl Acad Sci USA 1998; 95 : 1189-94.
The problem is that success stories like
Ribozyme have been too few.
Under your scenario, you can attach the
ribozyme, make it a better catalyst, and then it can be de-attached to go and be recycled elsewhere.
Second,
ribozymes acting on their own, without the help of proteins, are known to perform only a tiny set of simple reactions.
In contrast, achieving RNA interference using small interfering RNA is more efficient than Anti-sense oligos or
ribozymes. Recent studies were demonstrated that using siRNA is more rational as these molecules can induce selective silencing of exogenous viral genes in mammalian cells, and the process does not interfere with the recovery of cellular regulatory systems previously inhibited by viral gene expression (61).
To continue, there are four types of self-cleaving motifs: the hammerhead, the hairpin, the vsRNA, and the hepatitis delta
ribozyme. These four self-splicing introns catalyze reactions involving self-cleavage with a cyclic phosphate.
Joyce's specialty is the creation of
ribozyme systems that replicate and even evolve somewhat--on the verge of life, but not quite there.
Creating a retroviral expression vector for delivery of
ribozymes into eukaryotic cells.
We report a new class of highly efficient
ribozymes generated by directed in vitro evolution that can catalyze the aminoacylation from aminoacetyl CoA.
Expression of
ribozyme and antisense constructs targeted to the flax [Delta]9 desaturase.