re·verse tran·scrip·tion

reversal of the normal pattern of transcription (from DNA to RNA); the effective means is the viral enzyme reverse transcriptase.

Farlex Partner Medical Dictionary © Farlex 2012

reverse transcription

The process by which DNA is synthesized from an RNA template by means of the enzyme reverse transcriptase.

reverse transcription

the process of synthesizing complementary DNA from an RNA TEMPLATE, by the ENZYME REVERSE TRANSCRIPTASE. A PRIMER is required for enzyme activity. The process tends to be error-prone, because there is no EDITING of the newly synthesized DNA and therefore MUTATIONS can accumulate. Reverse transcription is a reversal of the normal flow of genetic information from DNA to RNA.see CENTRAL DOGMA.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

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References in periodicals archive

Reliability of reverse transcription polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells: a quality assurance initiative of the EORTC Melanoma Cooperative Group.

Variability of the reverse transcription step: practical implications

The qPCR reaction mixture comprised 12.5 [micro]L of 2x Maxima SYBR Green, qPCR Master Mix, 1.0 [micro]L of reverse transcription product, and 25 pmol of each realtime primer, made up to a final volume of 25 [micro]L with ultrapure water (Gibco BRL).

Development of strand-specific real-time RT-PCR to distinguish viral RNAs during newcastle disease virus infection

Characteristics of 24 patients with positive and negative results for infection with Zika virus, Brazil, 2015 Reverse transcription Mean (SD) No.

Zika virus outbreak, Bahia, Brazil

Reverse transcription was performed in a total reaction volume of 10 [micro]L at 55 [degrees]C for 30 min and then heat-inactivated at 85[degrees]C for 5 min.

Quantitative PCR measurement of tRNA 2-methylthio modification for assessing type 2 diabetes risk

Cells were then transformed with cDNAs generated by reverse transcription PCR reactions of total RNA isolated from skeletal muscles (soleus) of adult male rats exposed to and not exposed to the atrophy of simulated microgravity.

The effects of micro gravity induced cDNAs on the phenotypic expression of E. coli cells. (South Carolina Academy of Sciences Abstracts)

In this study, we examined the temporal expression pattern of AhR and its heterodimer partner, Arnt, during zebrafish development using reverse transcription polymerase chain reaction (RT-PCR).

Green fluorescent protein (GFP) as a marker of aryl hydrocarbon receptor (AhR) function in developing zebrafish (danio rerio). (Articles)

Reverse transcription PCR (RT-PCR) was conducted using a primer pair targeting nonstructural protein 1 (NSP1) of Getah virus (OneStep RT-PCR Kit, QIAGEN, Hilden, Germany) by using the RNA extracted from the blood samples (8).

Getah virus infection among racehorses, Japan, 2014

The trial investigated the variability of BCR-ABL1 testing by quantitative reverse transcription PCR (RT-qPCR) in 15 clinical labs that carry out routine monitoring of CML patients receiving tyrosine kinase inhibitors (TKIs) in five countries in Europe.

Asuragen, Labceutics disclose study data on standardisation of BCR-ABL1 testing in CML patients

Experimental procedures typically include sample-processing steps (i.e., extraction) and, when required, reverse transcription. If the volume changes and the efficiencies of these steps are accounted for, the most sensitive LOD theoretically possible can be expressed in units relevant to the experiment, such as copies per nanogram of tissue.

The MIQE guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Laboratory diagnostic techniques used included viral isolation, hemagglutinin inhibition serology, and multiplex, reverse transcription polymerase chain reaction.

Clinical diagnosis of flu highly accurate. (Disease watch)