re·stric·tion en·do·nu·cle·ase

one of many endonucleases isolated from bacteria that cleave or hydrolyze (cut) foreign double-stranded DNA chains at specific recognition sites defined by DNA sequences; these endonucleases have become standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences and are sometimes referred to as a "chemical knife," usually named by a three- or four-letter abbreviation of the name of the organism from which isolated (for example, EcoB from Escherichia coli, strain B).

Synonym(s): restriction enzyme

Farlex Partner Medical Dictionary © Farlex 2012

re·stric·tion en·do·nu·cle·ase

(rĕ-strik'shŭn en'dō-nū'klē-ās)

One of many endonucleases isolated from bacteria that hydrolyze (cut) double-stranded DNA chains at specific sequences, thus inactivating a foreign (viral or other) DNA and restricting its activity; standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences.
Synonym(s): restriction enzyme.

Medical Dictionary for the Health Professions and Nursing © Farlex 2012

restriction enzyme

restriction endonuclease

an endonuclease that recognizes a specific DNA base sequence (recognition sequence, recognition site, restriction sequence or restriction site) and cleaves both strands of DNA at or near that site. The enzyme cuts the DNA, generating restriction fragments with OVERHANGING ENDS or BLUNT ENDS. See also COHESIVE ENDS.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

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References in periodicals archive

It is likely that many of the 12 restriction endonucleases utilized did not yield differing haplotypes because these areas of the amplicon are highly conserved.

Mitochondrial DNA analysis of gene flow among six populations of collared lizards (Crotaphytus collaris) in West Central Texas

Restriction endonuclease cleavage maps of animal mitochondrial DNAs.

Evolutionary associations of brood parasitic finches (Vidua) and their host species: analyses of mitochondrial DNA restriction sites

Purified mtDNA was digested using 13 restriction endonucleases, under conditions suggested by the supplier (New York Biolabs, Inc.) for 7 h at 37 C.

Restriction fragment length polymorphisms in the largemouth bass (Micropterus salmoides salmoides) in a small Massachusetts kettlehole

Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites.

Genotyping of Staphylococcus aureus Strains Isolated from Bovine Mastitis in Turkey by using ERIC-PCR Method

The Stx-2A bacteriophages were highly similar in morphologic features, restriction endonuclease profiles, chromosomal integration sites, and superinfection immunity (2,3) and showed <65% similarity to Stx phages from non-O104 strains.

Shiga toxin 2A-encoding bacteriophages in enteroaggregative Escherichia coli O104:H4 strains

Generally, to produce sticky or complementary ends for sticky-end DNA cloning, insert DNA and vector are separately cut with same restriction endonuclease enzymes.

VECTOR CONSTRUCTION STRATEGIES FOR TRANSFORMATION OF WHEAT PLANT

A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data.

Chinese Holstein cattle shows a genetic contribution from native Asian cattle breeds: a study of shared haplotypes and demographic history

1A in the Data Supplement that accompanies the online version of this brief communication at http://www.clinchem.org/content/vol57/ issue5, genomic DNA is first digested by a restriction endonuclease (RsaI in this case) into fragments, one of which contains the entire target region between 2 closely spaced restriction sites.

Biotinylated probe isolation of targeted gene region improves detection of T790M epidermal growth factor receptor mutation via peptide nucleic acid-enriched real-time PCR

The amplified fliC gene was cleaved with HhaI restriction endonuclease (Invitrogen), when fliC(M) primers were used, and RsaI restriction endonuclease (Invitrogen), when fliC(F) primers were used.

Identification of putative new Escherichia coli flagellar antigens from human origin using serology, PCR-RFLP and DNA sequencing methods

Effect of the genotype for malignant hyperthermia as determined by a restriction endonuclease assay on the quality characteristics of commercial pork loins.

An improved method for characterization of the mutation associated to porcine stress syndrome by PCR amplification followed by restriction analysis/Um metodo melhorado para caracterizacao da mutacao associada a sindrome do estresse suino por amplificacao por PCR seguido de analise de restricao