The information content (IC) for the
recognition site is listed along with the DNA binding domain (DBD) of the TFs either the homeodomain (HD) or the C2H2 Zn fingers (ZNF).
In other words, the quick uptake can be attributed to many of the imprinted cavities situated on the surface of the outer shell MIPs, which make the
recognition sites available for template molecules resulting in shorter time to get the adsorption equilibrium [18, 22, 26, 37, 38].
Digestions using restriction enzymes with four base-pair
recognition sites were precipitated by ethanol before electrophoresis.
There was a
recognition site of Bc1I in CC genotype from the result of sequencing.
The DNA sequence for 5 amino acids (Asp-Asp-Asp-Asp-Lys) as a specific
recognition site was inserted between PTH gene sequence and the fusion partner DNA sequences.
Berzofsky, one ofseveral NIH workers involved in the project, a T-cell
recognition site newly discovered by the group apparently primes T cells to an earlier and higher immune response when later challenged with P.
The primers were designed by the Oligo 5.0 software, considering (when possible) the restriction endonuclease and the primer pair such that the PCR product contained a nonvariant
recognition site (i.e., a control digestion site), thus improving the reliability of the technique.
One portion of the adapter contains the
recognition site for FokI.
Type IIS restriction enzymes such as MmeI, FokI, and BstF5I cleave DNA outside the recognition sequence: MmeI, 20 bases 3' to the
recognition site on one strand and 18 bases from the
recognition site on the other strand, leaving a 2-base overhang protruding 3' end; FokI, 9 bases 3' to the
recognition site on one strand and 13 bases from the
recognition site on the other strand, leaving a 4-base overhang protruding 5' end; BstF5I, 2 bases 3' to the
recognition site on one strand and immediately 3' to the
recognition site on the opposite strand, leaving a 2-base overhang.