Detection, prevalence, purification and characterization of
lecithinase of Klebsiella pneumoniae.
(1959) The relation between the activity of a
lecithinase and the electrophoretic charge of the substrate.
Biochemical and morphological characterization: The three isolates were Gram positive rods,
lecithinase positive, showed haemolytic activity in blood agar and produced spores in oval way.
aureus appear as black colonies demonstrating
lecithinase activity as an opaque zone around the colony, encircled by a zone of clearing that is the result of lipase activity.
perfringens is a soil-borne Gram positive bacteria, is hemolytic, and produces the enzyme
lecithinase, and is anaerobic.
Different biochemical tests carried such as, fermentation reaction with different sugars, IMViC, H2S, Litmus milk reaction, Gelatin hydrolysis, Motility test, Egg yolk agar (
Lecithinase and lipase) (Rahaman et al., 2013).
Bacterial isolates collected from hot springs were screened for amylase, protease, lipase, gelatinase, cellulose, and
lecithinase activity (Figure 4).
perfringens alpha toxin (
lecithinase) on lecithin of egg yolk onto enriched egg yolk agar medium which appeared as pearly opalescence zone surround the colonies while this reaction was inhibited by C.
The positive colonies were subjected to biochemical test for identification such as catalase, coagulase, oxidase, indole, methyl red, Voges-Proskauer, Citrate, TSI, stormy clot fermentation,
Lecithinase, CAMP tests and sugar fermentation reaction as per Barrow and Feltham (1993).
All isolates were phenotypically identical; they produced broad halos of hemolysis and
lecithinase reactions and had positive glucose-1-phosphate acidification test results, reflecting the constitutive activation of the PrfA virulence regulon.