Pfizer and Amgen analyse the
glycan profile of every batch of Enbrel manufactured using a high-performance liquid chromatography (HPLC) method similar to the UPLC method applied by Miranda-Hernandez et al.
To record [sup.1]HNMR spectra we dissolve
glycan samples in [sup.2][H.sub.2]O to exchange labile [sup.1]H for [sup.2]H.
Therapeutic glycoprotein containing oligosaccharide chains (
glycans) covalently attached to amino acid side-chains plays a significant role in modulating yield, bioactivity, solubility, stability against proteolysis, immunogenicity, and clearance rate from circulation.
Effect of
glycan structure on physicochemical properties and functions of transferrin
Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called
glycans, is a fast-growing and important field of study in life sciences.
He joined NIBRT - The National Institute for Bioprocessing Research and Training, in 2007 based at the UCD Conway Institute of Biomolecular and Biomedical Research, representing NIBRT in the EU FP6 GLYFDIS - "
Glycans in Bodily Fluids, Potential for Disease Diagnostics" Consortium.
She theorised broad-range
glycan antibody assessments could one day become part of a routine patient exam, like blood levels of cholesterol and lipids, which provide early clues that something is amiss.
The
glycan profiles of urinary protein from DN and NDRD patients were analyzed by a lectin microarray as described previously.[13],[14] Briefly, the lectin microarray was blocked with a blocking solution containing 3% BSA for 1 h.
These variables are related to seven metabolic pathways, including TCA cycle, ubiquinone and other terpenoid quinone biosyntheses, N-glycan biosynthesis, other
glycan degradation, glycosaminoglycan degradation, and glycosylphosphatidylinositol (GPI) anchor biosynthesis and glycosphingolipid biosynthesis-ganglioseries.
To solve the problems, researchers have developed
glycan derivatization methods and ionic liquid matrices (ILMs).