For the study of the fragmentation patterns of HMPs, the following substances were analyzed: gal-1-P, glucose 1-phosphate, fructose 1-phosphate, and fructose 6-phosphate. Each substance was dissolved in mobile phase at 20 [micro]mol/L and was infused at a rate of 5 [micro]L/min into the tandem MS instrument.
However, there were marked differences in the relative abundance of fragments m/z 79 and 97, depending on the configuration of the HMP: The examined aldose monophosphates (AMPS; i.e., gal-1-P and glucose 1-phosphate) predominantly generate a fragment of m/z 79, whereas ketose monophosphates (KMPs; i.e., fructose 1-phosphate and fructose 6-phosphate) predominantly generate a fragment of m/z 97.