In the bacterium Clostridium kluyveri dihydrolipoyl dehydrogenase (synonymously called dihydrolipoamide dehydrogenase) BfmBC reduces Fe(III) complexes of citrate, ATP, and ADP in an NAD(P)H-dependent manner [2].
EsyPred3D also predicted the 3D structure of BfmBC using Geobacillus stearothermophilus dihydrolipoamide dehydrogenase crystal structure (PDB ID 1EBD) as the template (Table 3).
In Thermus scotoductus SA-01 a peripheral membrane-associated protein, identified as a dihydrolipoamide dehydrogenase (LPD) by sequence homology, has been shown to couple NAD(P)H oxidation to Cr(VI) reduction [16].
indiensis LPD1 Geobacillus stearothermophilus dihydrolipoamide dehydrogenase (1EBD) LPD2 Geobacillus stearothermophilus dihydrolipoamide dehydrogenase (1EBD) Trx Escherichia coli thioredoxin reductase (1CL0) NAD(P)H- Novosphingobium aromaticivorans ferredoxin nitrite reductase ArR (3LXD) reductase Tdr Mycobacterium tuberculosis thioredoxin reductase (2A87) Thioredoxin Hyperstable thioredoxin from Precambrian period (2YJ7) Indolepyruvate Pyrococcus furiosus 2-keto acid:ferredoxin ferredoxin oxidoreductase subunit alpha (1YD7) oxidoreductase C.
Semi-quantitative RT-PCR analysis of dihydrolipoamide dehydrogenase: Total extracted RNA was treated with RQ1 RNase Free DNase (Promega, California, USA) and semi- quantitative RT-PCR performed to analyze the dihydrolipoamide dehydrogenase gene expression.
The extracted RNA using method 1 had high integrity and was successfully used for cDNA synthesis, following the analysis of the differential expression for actin and dihydrolipoamide dehydrogenase genes from salt-treated and non-treated mangrove plants (Figs.
1 was of good quality due to the precise differential expression by dihydrolipoamide dehydrogenase and actin genes during Real time-PCR (Fig.
The present study focuses on dihydrolipoamide dehydrogenase, as an adapted mangrove gene to overcome salt stress effects.