Pullulanases are also known as debranching enzymes due to their capability to hydrolyze [alpha]-1,6 glycosidic linkages in starch and other branched polysaccharides (Van der Maarel et al., 2002; Hii et al., 2012).
Hyperthermophile, Pyrobaculum calidifontis, Debranching enzyme, Type II pullulanase, Amylopullulanase.
In the past, the metabolic role of debranching enzymes was initially thought to be the degradation of amylopectin in storage starch during germination, or in transitory starch.
(2003) and Tetlow (2006) concluded that amylose content in cereal was mostly controlled by granule-bound starch synthase (GBSS), whereas amylopectin generally was synthesized by the coordinated actions of soluble starch synthases (SS), branching enzymes (BE), and debranching enzymes (DBE).
The starch- debranching enzymes isoamylase and pullulanase are both involved in amylopectin biosynthesis in rice endosperm.
Key words: Rice (Oryza sativa L.), Starch debranching enzyme, Gene expression, Enzyme activity, High temperature
Meanwhile, high temperature at filling stage could also increase the amount of long B chains of amylopectin and decrease short B chains (Asaoka et al., 1985), which is more dominantly attributable to the changing activities of soluble starch synthase (SSS), starch branching enzyme (SBE) and starch debranching enzyme (DBE) in filling endosperms, rather than the limitation in sucrose supply from photosynthesis tissues and its cleavage metabolism in non-photosynthesis tissues.