The use of a retention ring for riboflavin application and protection of the
corneal limbus from riboflavin exposure protected LESCs and corneal epithelial cells against CXL-induced damage.
An injection was given below the
corneal limbus at the site planned so as to easily cover the ulcer.
The fascia of the
corneal limbus was pulled with surgical lines to maintain the cornea level.
After corneal images were recorded on day 14, all corneas were excised around the
corneal limbus and preserved in 4% paraformaldehyde at 4[degrees]C for 24 h.
The eye worm was removed under general anesthesia with surgical site at 5 o' clock on the
corneal limbus. Microscopic examination of eye worm revealed Setaria.
Microarray comparison of transcriptomes from laser-microdissected limbal and central corneal basal epithelial cells revealed the identities of about 100 genes that were specifically expressed in the
corneal limbus [21].