Amino Acid Sequence: The N-terminal
amino acid sequence of purified globin chains was deduced by Edman degradation in an automated sequencer; model Procise 491 (Applied Biosystems).
Based on such research, Fujitsu Laboratories, the Insight Centre, and Fujitsu Ireland have now included not only structural information about
amino acid sequences in the knowledge graph, but also chained information.
Sequencing of the PCR product and prediction of
amino acid sequence2004) and Expasy Bioinformatics Resource Portal (http://web.expasy.org.translate) for phylogenetic and
amino acid sequence analysis respectively.
In this study, evolutionary trace (ET) method which relies on both
amino acid sequence and structural information to analyze functional sites of proteins is used because of the advantage of having molecular and structural information in a single analysis 11].
The phylogenetic tree map was drawn by DNAMAN software on the basis of
amino acid sequence of GAPDH in 6 species.
An example of
amino acid sequence similarity resulting from sequence comparisons across biology is represented in Figure 1.
Scientists are creating these variants by modifying the
amino acid sequence of brazzein.
For example, 97 percent of the
amino acid sequence in human collagen matches that of the cow.
Table 1: ICH Topic Q6 B (Specifications: test procedures and acceptance criteria for biotechnological/biological products) Appendix for Physiochemical Characterization Structural characterization and confirmation a)
Amino acid sequence b) Amino acid composition c) Terminal
amino acid sequence d) Peptide map e) Sulfhydryl groups and disulphide bridges f) Carbohydrate structure Physicochemical properties --Molecular weight or size --Isoform pattern --Extinction coefficient --Electrophoretic patterns --Liquid chromatographic patterns --Spectroscopic profiles