The buffers and the 20 enzyme systems analyzed were; 1) Clayton & Tretiak (1972) buffer: Aspartate amino transferase (AAT; 2.6.1.1),
Aconitate hydratase (ACO; 4.2.1.3), Malic enzyme (ME; 1.1.1.40), Glycerol-3phosphate dehydrogenase (G3PDH; 1.1.1.8), Isocitrate dehydrogenase (IDH; 1.1.1.42), Malate dehydrogenase (MDH; 1.1.1.37), Glucose-6-phosphate dehydrogenase (6PGD; 1.1.1.49), Phosphoglucomutase (PGM; 5.4.
The strains isolated were typed by multilocus enzyme electrophoresis using eight enzymatic systems: malate dehydrogenase (MDH, EC1.1.1.37), isocitrate dehydrogenase (IDH, EC1.1.1.42) with substrate NAD and NADP, malic enzyme (ME, EC1.1.1.40), glucose-6-phosphate dehydrogenase (G6PDH, EC1.1.1.49), 6-phosphogluconate dehydrogenase (6GPDH, EC1.1.1.44),
Aconitate hydratase (ACON, EC4.2.1.3), and hexokinase (HK, EC2.71.1) [19].
We analyzed seven different enzyme systems:
aconitate hydratase (Acn), diaphorase (Dia), isocitrate dehydrogenase (Idh), malate dehydrogenase (Mdh), phosphoglucoisomerase (Pgi), phosphoglucomutase, (Pgm) and Triose-phosphate isomerase (Tpi).
Basal metabolism
Aconitate hydratase Lipase Fructose-1,6-biphosphatase F-II Fructose-bisphosphate aldolase Alpha-amylase B 3-oxoacyl-ACP reductase C-4 sterol methyl oxidase 2 Amine oxidase Scavenger receptor cysteine-rich protein Vacuolar H+-ATPase C chain 4.