Phenotypic and molecular characterization of plasmid mediated AmpC [beta]-lactamases among Escherichia coli, Klebsiella spp., and
Proteus mirabilis isolated from urinary tract infections in Egyptian hospitals.
Proteus mirabilis, Xanthomonas maltophilia and Salmonella enterica were also recovered from cadavers fixed with 10 % formalin.
Occurrence and detection of Amp C b-lactamases among Escherichia coli, Klebsiella pneumoniae, and
Proteus mirabilis isolates at a veterans medical center.
(6) 02 01 (16.6)
Proteus mirabilis (5) 03 -- Providencia spp.
The first was
Proteus mirabilis, a Gram-negative facultative anaerobic found in soil and water, while the second pathogen found was Pseudomonas aeruginosa that is a Gram-negative, multidrug-resistant pathogen associated with hospital infections.
Number tested (% Organism of total) ESBL AmpC MBL Citrobacter spp 1 (0.8) 1 (100) 0 0 Escherichia coli 55 (43.7) 22 (40.0) 7 (12.7) 15 (27.3) Klebsiella species 36 (28.6) 30 (83.3) 8 (22.2) 23 (63.9)
Proteus mirabilis 6 (4.8) 2 (33.3) 0 1 (16.7) Proteus vulgaris 5 (4.0) 2 (40.0) 0 1 (20.0) Providencia spp 6 (4.8) 4 (66.7) 1 (16.7) 1 (16.7) Alcaligenes spp 5 (4.0) 3 (60.0) 0 0 Pseudomonas aeruginosa 10 (7.9) 1 (10.0) 3 (30.0) 4 (40.0) Pseudomonas fluorescens 1 (0.8) 0 0 0 Acinetobacter species 1 (0.8) 0 0 0 Total 126 65 (51.6) 19 (15.1) 45 (35.7) Number in bracket: percentage.
shimperi were examined at a concentration of 20 [micro]g/mL against four pathogenic bacterial strains: one Gram-positive Staphylococcus aureus and three Gram-negative Escherichia coli, Klebsiella pneumoniae, and
Proteus mirabilis. Antibacterial potential of crude extract and isolated pure compound were assessed in terms of zone of inhibition of bacterial growth.
These isolates have been registered in GenBank under accession numbers (Klebsiella pneumoniae (KY049979), Klebsiella oxytoca (KY031321), Escherichia coli (MG198700), Pseudomonas aeruginosa (MG198702), Citrobacter freundii (MG198698), Acinetobacter baumannii (KY114514), Alcaligenes faecalis (MG198707), Enterobacter cloacae (KY205640), Bacillus horikoshii (MG198701), Staphylococcus aureus (KY176381), Staphylococcus haemolyticus (MG198699),
Proteus mirabilis (JF947362), Bacillus cereus (JX218990.1), and Shigella flexneri (KY199565)).
Initially, the stock cultures of Staphylococcus aureus, Salmonella typhi, Providencia alcalifaciens, and
Proteus mirabilis were revived by inoculating in broth media and grown at 37[degrees]C for 18 h.
The in vitro antibacterial activity of the prepared ligands and complexes were also evaluated against four bacterial strains e.g., Staphylococcus aureus, Bacillus subtilis, Escherichia Coli and
Proteus mirabilis, by the well-diffusion method.