After incubation, serum opsonized zymosan
(25 uL) and intracellular reactive oxygen species detecting probe (25 uL), luminol were added into each well, except blank wells containing only HBSS++.
-induced sterile sepsis was induced by intraperitoneal injection of zymosan
(Sigma Chemical Co.
To the "zymosan
" area of the slides a few drops of zymosan
reagent was added.
Innate immune cells carry special molecules called Toll-like receptors (TLRs) on their surface that recognize and bind highly conserved structures on bacterial, fungal, or viral surfaces, including peptidoglycan, flagellin, zymosan
, and lipopolysaccharide (LPS, also known as endotoxin) (Janeway and Medzhitov 2002).
quando estes foram estimulados por zymosan
opsonizado ou PMA.
The first of these is inflammation, which the scientists induce by injecting a substance called Zymosan
in an area just behind the edge of the iris of the mouse's eye.
This is in accordance with similar data demonstrating analgesic and anti-inflammatory effects of various Hypericum species in mice models either with tetradecanoylphorbol acetate (TPA) induced ear inflammation (Rabanal 2005, Sanchez-Mateo 2006), zymosan
induced organ dysfunction syndrome or of carrageenan induced pleuritis (Sanchez-Mateo 2006, Di Paola 2007).
Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan
and acetic acid in mice.
The pineal gland hormone melatonin improves survival in a rat model of sepsis/shock induced by zymosan
Se pueden activar diferentes TLR's con una misma molecula, por ejemplo, el zymosan
puede activar TLR2 y TLR6 al mismo tiempo.
Modulation of zymosan
and phorbol ester stimulated respiratory burst of rock bream (Oplegnathus fasciatus) phagocytes by staurosporine, genistein and sodium orthovanadate.
A solucao de vermelho fenol (Sigma), para os ensaios de producao de H2O2, consiste de 5,5 mM de dextrose, 0,56 mM de vermelho fenol e 8,5 U/mL peroxidase "horseradish" (Sigma) em PBS pH 7,4 a 340 mOsm, e previamente adiciona-se 0,05% de zymosan
(2,3 x 108 particulas/mL - Sigma), para os ensaios de fagocitose.