zymogram


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zy·mo·gram

(zī'mō-gram),
Strips of paper, gels, etc., in which the locations of enzymes, separated electrophoretically or by other means, are demonstrated by histochemical methods.
[zymo- + G. gramma, something written]
Farlex Partner Medical Dictionary © Farlex 2012

zymogram

(zī′mə-grăm′)
n.
A strip or band of electrophoretic medium showing the pattern of enzymes or isoenzymes after their separation by electrophoresis.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

zymogram

(zī′mō-grăm)
An electrophoretic graph of the separation of the enzymes in a solution.
Medical Dictionary, © 2009 Farlex and Partners
References in periodicals archive ?
Allele frequencies and their variances were calculated from genotype frequencies observed in zymograms using the BIOSYS-1 package (Swofford and Selander 1989).
The study of inhibitors both in vitro and through the zymogram in adults of M.
The SDS-PAGE analysis of anion-exchange fractions revealed a single protein band of approximate 25 kDa as shown in Figure 2A, lane 4, that corresponds to the only active band seen on casein zymogram, presenting the same molecular weight (Figure 2B).
Zymogram of both testicular samples revealed only single band at identical position.
For the protease zymogram, the proteins were first separated in nondenaturing conditions on IEF (3,0-9,0) Phastgels.
Apart from the inability of the zymogram technique to detect all variation (allozyme bands effectively are electromorphs, that is, collections of similarly charged enzyme molecules that differ in their amino-acid sequence at one site at least; King and Ohta 1975), a given Taraxacum genotype may comprise a heterogeneous collection of individuals sharing an identical three-locus electrophoretic profile.
For activity staining (Zymogram) of xylanase by SDS-Xylan-PAGE, SDS was removed by washing the gel at room temperature in solution-A (50 mM [Na.sub.2]HP[O.sub.4], 50 mM Na[H.sub.2]P[O.sub.4] (pH 7.2), isopropanol) for ne hr and solution-B (50 mM [Na.sub.2]HP[O.sub.4], 50 mM Na[H.sub.2]P[O.sub.4] (pH 7.2)) for one hr, respectively.
Zymogram differences among the five species of the abalone from the coasts of Japan.
Cell culture media, antibiotics, and 10% Zymogram gel were purchased from Life Technologies (Rockville, MD, USA).
Additionally, we have improved the protocols of electrophoresis, traditionary chromosomal preparation and exogenous gene transfection by trying a local apparatus, different buffers, colchicine and hypotonic time, plasmid DNA incubation time and lipofectamine combination, thus make these techniques feasible under our conditions and greatly improve isoenzyme zymogram, karyotype quality and exogenous gene transfection efficiency.