Stage II (b) consists of type A spermatogonia (A), leptotene spermatocyte (L), zygotene spermatocyte (Z), pachytene spermatocytes (P), elongating spermatids (E), and Sertoli cells (S).
Stage V (a) shows zygotene spermatocytes (Z), pachytene spermatocytes (P), round spermatids generation 1 (Rj) and generation 2 ([R.sub.2]), elongated spermatids (E), and Sertoli cells (S).
4: Effects of CBZ, IPR and CCK on the chronology of meiosis Primary spermatocytes stained with an anti-SCP3 antibody were selected to evaluate the respective percentages of leptotene, zygotene
, pachytene and diplotene stages of the meiotic prophase.
Microscope analysis: Nuclei of the first meiotic prophase at leptotene, zygotene and pachytene stages were analyzed using a Zeiss Axioskop epifluorescent photomicroscope equipped with propidium iodide, fluorescein isothiocyanate, and 4,6-diamidino-2-phenyllindole filters (Carl Zeiss, MC80, Oberkochen, Germany) equipped with an epifluorescent system.
Statistical analysis: Mann-Withney test was used in order to evaluate the frequencies of leptotene, zygotene, and pachytene stages, and the staging of pachytene cells in controls and patients.
Respective frequencies of leptotene, zygotene and pachytene stages: In the controls, the frequency of leptotene stage (Fig.1A-B) varied from 1 to 4.50 per cent with a mean of 2.30 per cent, the frequency of zygotene stage (Fig.1C) varied from 0.50 to 2.50 per cent with a mean of 1.45 per cent, while the frequency of pachytene stage (Fig.
In patients, the frequency of leptotene stages varied from 2 to 11.50 per cent with a mean of 7.95 per cent, the frequency of zygotene stages varied from 3.50 to 23 per cent with a mean of 9.75 per cent, and the frequency of pachytene stages varied from 57 to 92.50 per cent with a mean of 75.30 per cent (Table II).