Sputum samples were obtained from 211 of the 286 patients and sent to the laboratory of China CDC (Beijing, China) for Legionella culture, which used both buffered charcoal yeast extract agar
and buffered charcoal yeast extract agar
supplemented with Legionella GVPC (glycine, vancomycin, polymyxin, cycloheximide) Selective Supplement (Oxoid, https://www.thermofisher.
This paper present the morphological properties of Aspergillus terreus and Aspergillus versicolor including the conidiogenesis process when cultured on malt extract agar and yeast extract agar
medium as a common medium used for identification of aspergilli.
Subsequently, the organism was sub-cultured for identification on other bacteriological media, including glucose malt extract, yeast extract agar
, tryptic soya agar and Lowenstein-Jensen media.
Fungal isolation, colony and cell morphology for fungal identification were done by using Malt Extract Agar (MEA), Potato dextrose agar (PDA), Czapek yeast extract agar
(CYA), and Sabouraud dextrose agar (SDA) media, supplemented with 0.05% chloramphenicol (Khan et al., 2014; Nonaka et al., 2011).
Water Plate Count Agar (ISO) and Yeast Extract Agar
, for example, deliver the total viable counts needed when monitoring ground water integrity, water treatment processes and water supplies for food and beverage preparation, while R2A Medium fulfils the same function for heterotrophic bacteria.
The tests included the fermentation of sugars, liquid assimilation of carbon compounds, liquid assimilation of nitrogen compounds, growth at 37'C, growth on 50% (W/V) glucose yeast extract agar
, growth in vitamin--free media, growth in media containing 16% NaCl, resistance to 0.01% cycloheximide, and urease activity.
For the identification of Aspergillus isolates to species level, three point inoculation technique was performed on three differential media; MEA, Czapek yeast extract agar
(CYA), and Czapek Yeast Extract agar
supplemented with 20% sucrose (CY20S) all prepared according to the recipes described by Klich and Pitt .
This easy, rapid method produces results that correlate with the International Standard IS0 6222 Yeast Extract Agar
pour plate method.
In ME-YEA yeast-extract (0.4 g/l) was amended in malt extract agar media; in PDA-PS-YEA peptone (2 g/l), yeast-extract (2 g/l) and sucrose (2 g/l) were amended in potato dextrose media; in YE-RB-SA sucrose (15 g/l) and rose-bengal (2 g/l) were amended in yeast extract agar
. After sporulation it was identified based on morphological characters using manuals of Nagamani et al.
We then inoculated 0.1-mL samples onto buffered charcoal yeast extract agar
(Becton Dickinson, Sparks, MD, USA) supplemented with glycine, vancomycin, polymyxin B, and cycloheximide (Oxoid, Basingstoke, UK).