The widespread use of yeast artificial chromosomes
as cloning vectors, has created a demand for reliable method to recover intact chromosomal DNA from preparative pulsed field gels.
(In 1996 Resnick, with NIEHS colleagues Vladimir Larionov and Natalya Kouprina, perfected a method using yeast artificial chromosomes
, or YACs, for the specific isolation of entire human genes.) In another, changes are introduced into homologous genes or cloned genes in model systems.
A DNA contig of this region spanning 2,400 kb was constructed from large-capacity yeast artificial chromosomes
and P1 bacteriophage clones (76).
Although investigators have made yeast artificial chromosomes
for more than a decade (SN: 6/5/93, p.