Therefore, orange oil would be a suitable alternative solvent to promote gutta-percha dissolution compared with potentially toxic solvents such as xylol
After dewaxing in xylol
and rehydrating through graded alcohols to tap water, endogenous peroxidase activity was blocked using 1% hydrogen peroxide (Cameron Chemical Consultants, Cape Town, South Africa) solution in methanol for 15 minutes.
Cross-sectional cuts were made through the middle portion of the sampled gonads, and then dehydrated in alcohol, at concentrations of 80%, 90%, and 100%, cleared in xylol
, and embedded in paraffin blocks.
Entre los solventes organicos que son usados con mayor frecuencia en endodoncia tenemos: cloroformo, xylol
, halotano, eucalyptol, turpentine, y aceite de naranja.
Hydrocarbon solvents like naphtha or xylol
(paint thinner) are effective for removal of oil or grease but typically do not evaporate as rapidly as alcohol, and pose greater health risks.
Se hizo un lavado con hematoxilina, lavados con agua corriente hasta que salio la coloracion, pases por alcohol acido al 0,4%, agua destilada, agua amoniacal y agua destilada; se deshidrato con concentraciones ascendentes de etanol, dos lavados con xylol
y se hizo su montaje final con entellan.
Slices were incubated in three changes of 100% xylol
for 5 min at room temperature and these were dehydrated in an ethanol series of 100%, 85%, 70% and 50% 3 min each at room temperature.
After automated immunostaining, slides were washed with Dako buffer, rehydrated in graded alcohols and fixed with Xylol
The dehydrated antennae were rinsed in xylol
for 10 min, then put individually in Canadian balsam and observed under a light microscope (AO Microstar, American Optical Co.
Briefly, stained microscopic preparations were washed in xylol
and absolute ethanol, scraped with a sterile blade, and collected in a microcentrifuge tube in 1 x phosphate buffer.
Table 2 -- Magnesium-Rich Primer Materials Formulation Materials (A) Hyb-E23A Desmodur[TM] E23-A, Aerosil R202, Eckagranules[TM] PK51/31, Epon[TM] 1001 CX, Propylene Carbonate/EEP (B) MC-PUR (27) Desmodur E23-A, Bentone[R] 34, Eckagranules PK51/31 Anti Terra[R]U, Aromatic solvent Xylol
(C) Hyb-N3300 Desmodur NC-3300, Aerosil[TM] R202, Eckagranules PK51/31, Epon[TM] 1001CX, Propylene Carbonate/EEP (D) Epoxy-Polyamide Epon 828, Epicure[R] 3115, Aerosil R202, Eckagranules PK51/31, AntiTerra[TM]U, Aromatic solvent Xylol
Table 3 -- Viscoelastic Properties of Polymer Films Crosslink Density Polymer Film Tg ([degrees]C) (mol/[cm.
Brevemente, la muestra se deshidrato con una gradiente ascendente de alcoholes y xylol
, se incluyo en parafina histologica.