Positive whiff test
: fishy odour of vaginal discharge before or after addition of 10% KOH.
(Amine test) helps in making diagnosis through ammonical odour (fishy odour).
Third swab was used for smear, pH test, whiff test
, and KOH mount the swab was then mixed with 2 drops of 10% potassium hydroxide on a slide and immediately held close to nose to detect the fishy odour associated with volatile amines (Whiff amine test).
Awareness can be lifesaving when accompanied by practical precautions such as upgrading the kitchen smoke detector, looking at expiration dates of refrigerated foods rather than relying on the "whiff test
," drinking water on a schedule, and flavoring with spices instead of sweeteners.
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In this situation if vaginal pH test and Whiff test
are performed, definite conclusion can be drawn and proper treatment can be given.
So ask for Thornback, and don't be afraid to take the whiff test
A positive whiff test
(mixing vaginal secretions with a 10 percent KOH solution and noting a fishy smell) and a pH > 4.5 on the vaginal secretion lead to a fairly definitive diagnosis of bacterial vaginosis.
The four diagnostic elements are: A vaginal fluid of pH>4.5, presence of "clue cells" (Epithelial cells with unclear borders, dotted with bacteria) on microscopic examination of vaginal swab samples in normal saline, milky homogeneous, adherent vaginal discharge and a positive 'whiff test
, which is accentuation of an amine or 'fishy' odor of discharge after addition of 10% potassium hydroxide.
Positive amine test (Whiff test
) - vaginal discharge collected from the posterior fornix of vagina was swabbed onto a glass slide followed by the addition of 10% KOH.
. The whiff test
is performed by adding drops of 10% potassium hydroxide solution to the vaginal fluid.
Other laboratory signs include a vaginal pH greater than 4.5, a positive whiff test
(fishy odor on addition of 10 percent KOH to vaginal secretions), and abnormal vaginal flora (absence of lactobacilli and increase of Gardnerella and anaerobes).