* A review of the
Western Equine Encephalitis products under development by companies and universities/research institutes based on information derived from company and industry-specific sources
Despite these uncertainties, investigations showed that mosquitoes transmitted virus when their body titers exceeded 4-5 logs of virus for EEEV (23-25) and
western equine encephalitis virus (38,39).
During acclimation, feral rats were determined to be seronegative for EEEV, VEEV, and
western equine encephalitis virus by 80% plaque-reduction neutralization tests ([PRNT.sub.80]), and they were screened by immunofluorescent assay for persistent infection with Bayou (Hantavirus) and Whitewater Arroyo viruses (Arenavirus), known to be enzootic in the region.
In response to the anticipated arrival of WNV, the state of Colorado added WNV serologic testing to its existing sentinel chicken surveillance (which included serologic testing for
western equine encephalitis and St Louis encephalitis), and initiated surveillance of mosquito pools, dead birds, and equines.
Overall, 4% of the dog sera we tested contained neutralizing EVEV antibodies, a rate similar to the seroprevalence for the major human pathogenic arboviruses (EVEV, EEEV, Saint Louis encephalitis virus,
Western equine encephalitis virus) in Florida detected in >2,500 sera from wildlife in 38 of the state's 67 counties from 1965 to 1974 (24).
Negative results were also obtained for the alphaviruses
Western equine encephalitis, Eastern equine encephalitis, and Venezuelan equine encephalitis by using mouse hyperimmune ascitic fluid.
