Histological findings: a total of 11 samples were processed, staining with hematoxylin and eosin, Masson's trichrome stain and von Kossa stain to differentiate the osteoid from the mineralized bone.
11, 29, 37, 38) The samples subjected to Masson's trichrome stain and von Kossa stain showed similar results in both groups, suggesting the presence of new bone formation in both groups (figures 10 and 11).
Special stains including Periodic acid Schiff (PAS) with diastase, Perl's stain, von Kossa stain
were applied which highlighted MG bodies (fig-1b,c,d,e).
Michaelis-Gutmann bodies stain blue with Haematoxylin-eosin and are positive for calcium and iron with the von Kossa stain
and the Perl's stain, respectively.
3) Michaelis-Gutmann bodies can be intracellular or extracellular and can be visualized by von Kossa stain
for calcium or Prussian blue stain for iron (Figure 3).
Three slices were cut from each group; each slice was placed on its own microscope slide and dyed as follows: a) hematoxylin and eosin stain (this process was conducted at the Laboratory of Universidad de Antioquia School of Dentistry); b) Masson's trichrome stain and c) von Kossa stain
(the last two at the Institute of Pathology of Hospital Universitario San Vicente of Paul, HUSVP).
With von kossa stain
, calcium deposits are found in approximately 75% of the tumours.
Von Kossa stain
demonstrated calcified fragments within the circular structures.
Histopathologic findings revealed fragmented and calcified elastic fibre in dermis visible under haematoxylin-eosin stains and which is more evident in specific elastic fibre, von Kossa stains
(Figure 3, 4 and 5).
Some of the histiocytes had basophilic intracellular MG bodies (Figure 1), which were round bodies of varying sizes that reacted positively with the periodic acid-Schiff and von Kossa stains