Some slides were also prepared and stained with von Kossa for the detection of the presence of microcalcifications within the tumor using a von Kossa Stain
Kit (IHC World).
The results of histochemical testing of the rosette crystals using von Kossa stain
and Pizzolato's method were consistent with previously reported staining characteristics of calcium oxalate crystals [1, 2].
* Von Kossa stain
: Percentage of osteoid (pink stain) and mineralized bone (dark black or brown stain).
Special stains including Periodic acid Schiff (PAS) with diastase, Perl's stain, von Kossa stain
were applied which highlighted MG bodies (fig-1b,c,d,e).
Case###Location###Iron Perl's Stain###Copper Rhodanine Stain###Calcium - Von Kossa Stain
Histopathological examination would show a focus of a well-circumscribed, round, basophilic substance, which would stain black with the Von Kossa stain
. It may be located in the upper dermis, surrounded by thick collagen fibers and sometimes by epithelioid and multinucleated giant cells .
While frank calcific changes could not be identified in the breast cores by either hematoxylin and eosin stain or Von Kossa stain
, the histologic pattern of extensive ischemic necrosis was felt compatible with the clinical setting of systemic calciphylaxis involving the substance of the right breast, and the immunohistochemical studies helped to further exclude an entirely necrotic malignancy.
Michaelis-Gutmann bodies stain blue with Haematoxylin-eosin and are positive for calcium and iron with the von Kossa stain
and the Perl's stain, respectively.
(3) Michaelis-Gutmann bodies can be intracellular or extracellular and can be visualized by von Kossa stain
for calcium or Prussian blue stain for iron (Figure 3).
Analysis of von Kossa stain
positive areas showed that hMSC seeded on CGCS, and cultured under differentiation conditions for 14 days, 0.28% (mean, SD= 0.03%, n=3) of the image area was covered by calcium deposits.
Paraffin-embedded liver sections (5 [micro]m) were stained with hematoxylin-eosin (HE) to evidence necrosis and cellular inflammation or with von Kossa stain
to evidence calcifications.
Three slices were cut from each group; each slice was placed on its own microscope slide and dyed as follows: a) hematoxylin and eosin stain (this process was conducted at the Laboratory of Universidad de Antioquia School of Dentistry); b) Masson's trichrome stain and c) von Kossa stain
(the last two at the Institute of Pathology of Hospital Universitario San Vicente of Paul, HUSVP).