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They used a device called Von Frey Hairs to gauge whether participants could discriminate between different amounts of force applied to their tongues.
Thus, the ability to perceive touch applied with von Frey hairs decreases with age as has been shown in several large studies with quantitative sensory testing [48, 49].
Abbreviations CIMT: Constraint-induced movement therapy D2: Index finger GOT: Grating orienting task [DELTA]GOT: Difference in grating orientating task (posttest value--pretest value) SSDT: Shape-sorter-drum task [DELTA]SSDT: Difference in shape-sorter-drum task (posttest value--pretest value) t1: Baseline evaluation t2: Treatment evaluation TFD: Temporary functional deafferentation VFHT: von Frey hair testing VFHT-D2: von Frey hair testing at the index finger [DELTA]VFHT-D2: Difference in von Frey hair testing at the index finger (posttest value--pretest value) VFHT-FA: von Frey hair testing at the forearm [DELTA]VFHT-FA: Difference in von Frey hair testing at forearm (posttest value--pretest value) n.s.: Not significant.
For mechanical allodynia, we measured the withdrawal threshold of the tail in response to a series of von Frey hairs (log units: 3.61, 3.84, 4.08, 4.31, 4.56, 4.74, 4.93, and 5.18; equivalent in bending force to 0.4, 0.6, 1.0, 2.0, 4.0, 6.0, 8.0, and 15.0 g; Stoelting, USA).
On day 0, a tail immersion test or von Frey hair test was performed and then oxaliplatin (6 mg/kg) or vehicle (5% glucose solution) was intraperitoneally injected, followed by an oral administration of GBT or saline.