In spite of some reports regarding the adverse effects of oocyte cryopreservation (40-44), there was poor information related to the changes in mtDNA copy number and mitochondrial enzyme activity of oocytes after vitrification
. Therefore, the object of this study was determining the alterations in mtDNA copy number, ROS level, mitochondrial enzyme activity, TFAM gene expression and mitochondrial distribution in vitrified oocytes compared with non-vitrified group.
The cryopreservation of lateral buds was successfully obtained with the encapsulation vitrification
technique, using PVS2 incubation for 15 minutes before plunge into LN.
The MSCs were expanded up to passage 5 and subsequently subjected to vitrification
and global gene expression examination as described below.
Two techniques are used in cryopreservation: slow freezing and vitrification
. Pioneering studies on mammalian embryo freezing were reported in 1972 [23, 24].
In this study, we explored the effects of L-proline on mouse oocyte vitrification
In this follow-up work, we investigate how the modulation of autophagy with rapamycin during vitrification
affects the rates of vitrification-warming and IVF and development.
These include vitrification
(MARKOVIC et al., 2013; GANINO et al., 2012; MATSUMOTO and SAKAI, 2003; WANG et al., 2003a), encapsulation-dehydration (MARKOVIC et al., 2013; BAYATI et al., 2011; WANG et al., 2003a; WANG et al., 2000), encapsulation-vitrification (GRIBAUDO et al., 2012) and droplet vitrification
(PATHIRANA et al.
Their safe and compliant work demonstrates their deliberate and methodical approach to decommissioning, said Dan Sullivan, EM WVDP federal project director for the Vitrification
hombroniana shoot tips to cryopreservation using vitrification
A small system that could be set up next to individual waste storage tanks has been proposed as a demonstration project for the preparation of waste to feed to the vitrification
plant in its initial years of operation.
The effect of solid-liquid demixing, liquid-liquid demixing, and vitrification
on morphology of polylactide membranes has been investigated by Witte at al.
The Camel Reproduction Centre is pleased to announce the birth a dromedary camel calf born after successful vitrification
(freezing) and thawing of a 7 day old camel embryo.