viral transport medium


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viral transport medium

Abbreviation: VTM
A nutrient substance (usually a buffered liquid) used to carry and maintain the viability of specimens to a microbiology laboratory for identification and analysis of disease-producing viruses.
See also: medium
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Thus, subconfluent Vero E6 cells (ATCC CRL-1586) were inoculated with 500 [micro]L of viral transport medium containing a cotton swab impregnated with urine or pharyngeal epithelial cells, as previously described (3).
Each swab was collected in 1 mL viral transport medium (1x Hank's balanced salt solution containing 5% fetal bovine serum, penicillin [100 U/mL], and streptomycin [100 [micro]g/mL], pH 7.
Briefly, viral RNA was extracted from 140 [micro]L clinical specimens in phosphate-buffered saline or viral transport medium with a viral RNA mini kit (Qiagen, Hilden, Germany) and a final elution with 60 [micro]L AVE buffer.