RetroNectin reagent enables the effective viral transduction
of gene products into T lymphocytes and the expansion of naive T-cell populations in culture.
the expansion of hematopoietic stem cells from cord blood for allogeneic transplantation and enhancement of viral transduction
efficiency for autologous gene therapy to treat rare diseases.
pRUF is an IRES-GFP vector and GFP works as a reporter gene for monitoring and evaluation of viral transduction
 tested a variety of polycations and determined that, depending on the specifics of the cell population, no cation can be claimed to be superior at enhancing viral transduction, and studies should test multiple variants to determine optimal performance.
While viral transduction and electroporation-based approaches have resulted in high efficiencies of gene expression in NK cells, these methods can be laborious and require specialized equipment and laboratory setups.
Caption: FIGURE 3: Functional assessment of HiPSC-ECs with or without viral transduction
Moreover, as E1B-55K is also involved in the translocation of nuclear viral mRNA to ribosomes and ONYX-015 is E1B-55K-null, the replicative potential is attenuated and indeed viral transduction
in glioma models has not shown any substantial amount of oncolytic activity47,49.
Viral transduction dynamics and viral titre prediction: the MATLAB R2012a software (MathWorks, Natick, MA, USA) was used to predict the viral transduction dynamics (f[(x).sub.td]) and viral titre (f[(x).sub.vt]) for the analysed plasmids.
The analysis of the viral transduction rates revealed that both untreated or DMSO-treated cells experienced minor decreases in transduction rates for the pMD9-based plasmids with different cPPT modifications in comparison to pMD9 and pWPXL controls (Figures 4(a) and 4(b)).
Using a technique called viral transduction
, they then introduced DNA that codes for sodium channels into the ESC-derived OPCs.
The scientists used immortalised liver cells without the ability to perform metabolism, and applied viral transduction
to "infect" the cells with viruses that have specific drug-metabolising genes leading to the expression of drug-metabolising enzymes.
with a Modified Culture Procedure.