Results: As results, E2 and BPA upregulated the protein expression of vimentin
, cathepsin D, and MMP-2, but downregulated the protein expression of E-cadherin via ER signaling pathway, suggesting that E2 and BPA promote EMT and cell migration related gene expressions.
In the immunohistochemical analysis, the cells exhibited diffuse membranous positive staining with CD99 and vimentin
, while cytokeratin was negative (Fig.
In general, immunohistochemical studies show positivity with vimentin
, CD68, CD 10, and other nonlineage markers.
Tenders are invited for Supply of Immunostatins Vimentin
,C020, cd3, amb 45 etc.
To determine whether germ cell aggregation in rats resulted from impaired Sertoli-germ cell interaction at the cell membrane, we performed double fluorescence immunohistochemistry for N-cadherin plus vimentin
and for APC plus espin; results were analyzed using confocal laser scanning microscopy.
Cytokeratin and vimentin
stains showed positive expression in the neoplastic cells within the mass.
This cellular interaction was associated with increased expression of adhesion and metastatic molecules, active cell cycle proteins, transforming growth factor-beta 3 (TGF-|33), and vimentin
, which is a marker of epithelial to mesenchymal transition (EMT).
The tumor cells were immunohistochemically reactive for CK7 and were negative for vimentin
Rich in sulfated galactans derived from the Hypnea musciformis algae, this novel ingredient is said to protect the cytoskeleton by maintaining vimentin
organization and preserving synthesis of the extracellular matrix proteins; as well as support the matrix network by stimulating the formation of collagen and fibrillin networks.
Immunohistochemical studies were undertaken using antibodies CK7 (1:750 Dako), cytokeratin AE1/AE3 (1:1000 Dako), EMA (1:750 Dako), CD10 (1:300 Novocastra), vimentin
(1:4000 Dako), S100 (1:4000 Dako), TTF-1 (1:250 Novocastra) and thyroglobulin (1:500 Dako).
Immunohistochemically, the tumour cells were strongly positive for vimentin
(Figure 1E) and focally positive for desmin (Figure 1F).
Yapilan immmtinohistokimyasal incelemede kollajen demetlerinde vimentin
ve myofibroblastik hucreterde ise duz kas aktini (SMA) pozitifligi goziendi (Realm 2, 3).