Immunohistochemistry stain showed that vimentin
and smooth muscle actin were positive in all cases of desmoplastic fibroblastoma.
Neuron specific enolase (NSE), CD56, vimentin
, progesterone receptor (PR) and [beta] catenin were performed on 8,5,3,13 and 16 cases respectively and showed positivity in majority of cases.
, ARK5, and GAPDH primary antibodies for western blotting were purchased from Abcam (USA).
A screening panel consisting of cytokeratin (CK), S100, vimentin
, and leukocyte common antigen (LCA) should be applied first as a general approach to a tumor of unknown origin, as illustrated in Figure 1.
, glycoprotein, SMA, desmin, and dimentin (leu-7).
To further determine the cell line of origin, immunohistochemical labeling for S100 protein (rabbit polyclonal anti-SlOO at 1:100, Novocastra, Newcastle, UK), CD34 (goat polyclonal anti-CD34 antibody at 1:80, Santa Cruz Biotechnology, Santa Cruz, CA, USA), smooth muscle actin (SMA; mouse monoclonal anti-SMA antibody at 1:100, Dako, Carpinteria, CA, USA), and vimentin
(mouse monoclonal antivimentin antibody ViM3b4 at 1:50, Dako) was performed on 5-pm serial sections of the mass as follows: brain for S100 protein; uterus for SMA; haired skin for vimentin
; and hemangiopericytoma for CD34.
Neuroblasts were positive for vimentin
and S100 (Figure 1E), specifically in the nucleus and cell membrane, and exhibited discrete positive staining for NSE in the cytoplasm (Figure 1D).
Immunohistochemically, the tumour cells showed a strong positive staining with CD34, Bcl-2, and vimentin
Biomarkers such as keratins, vimentin
, S100protein, CD45, CD3, CD20, and CD30 are popularly used for cancer diagnostics in lab tests.
Immunohistochemistry was performed with markers like keratin, vimentin
and HMB-45 to know the histogenesis of tumour.
Immunohistochemistry revealed positive staining with S-100 protein, Vimentin
, smooth muscle actin (SMA) and negative staining for Estrogen and Progesterone Receptors.
7,8) Markers associated with malignant melanoma include S-100 protein, gp 100, MART-1, tyrosinase, NK1/ C3, vimentin
, HMB-45, and KBA.