of sperm swimming in jelly of Xenopus laevis eggs and in Fl were obtained as described in Reinhart et al.
Observation of cyst formation by time-lapse video microscopy
The biggest problem in a video microscopy
system is the lack of a high-resolution "The performance of color cameras and even color monitors has leapt ahead of that for hardcopy printers," says Nikon's Shuett.
Second, video microscopy
of mechanically-ruptured, fertilized D.
The facts that video microscopy
did not reveal all cells in the strands to be moving, and that not all cells in the strands are elongate, could also be taken as evidence against the transport hypothesis.
Finally, bright field video microscopy
has high temporal resolution (30 frames/s video rate) for quantifying rates of action of gossypol on sperm.
In addition, the primary gravity response, fixing the polarity of nuclear migration and rhizoid growth, occurs in a relatively synchronized population of cells relatively soon after light-induced initiation of germination, and it can be visualized easily through the clear spore coat of Ceratopteris with video microscopy
equipment already developed and used in previous shuttle experiments (STL-B).
1D), long microtubule bundles and individual microtubules lie parallel to each other, indicating that the procedure does not significantly perturb their original organization, as defined by fluorescence (1) and video microscopy
In aequorin-labeled cells the individual [Ca.sup.2+] events are seen as bright observable blobs (BOBs; defined in 13-15) when viewed with a photon-counting video camera and video microscopy
techniques (e.g., 13-15).
Alternatively, for video microscopy
, the dissociated tissue was briefly pelleted at 1000 x g for 15 s to remove spicule debris, and the top of the pellet was pipetted onto a coated coverslip in a dish of seawater.
We also described a method for very rapidly obtaining serial optical sections, repeatedly in time lapse, using video microscopy
combined with synchronous computerized control of the objective and condenser fine-focus controls (4).
The blastomeres were injected with 10 pl doses of semisynthetic aequorin (16) shortly after the first cytokinesis following fertilization, and observed by multispectral video microscopy
(5-11) in the dark at 14 [degrees] C.