Pertains to the ability of the compound O/129 to inhibit the growth of Vibrio species; vibriostatic agent O/129 helps distinguish Vibrio species that are susceptible to the agent from closely related species that are resistant.
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The cholera strain showed resistance to three types of antibiotics: nalidixic acid, and nitrofurantoin, vibriostatic agent O129 .
The biochemical tests were done using commercially available API20E (Biomerieux[C], France) in combination with oxidase, catalase and vibriostatic test.
Sensitivity was demonstrated with the vibriostatic gent, 0/129 (150 [micro]g/disc and 10 [micro]g/disc).
Sensitivity was demonstrated with the vibriostatic agent 0/129 (150 [micro]g/disc and 10 [micro]g/disc).
Furthermore, Aeromonas's resistance to vibriostatic compound O/129 (150 pg) and the variable presence of ornithine decarboxylase activities differentiate the genus from the oxidase-positive Enterobacteriaceae-like organisms, Plesiomonas, and Vibrio.
Standard count agar (tSa, Oxoid, UK) for THB and thiosulphatecitrate-bile sucrose (TCBS) agar (Oxoid) as well as sensitivity to the vibriostatic agent (0/129) (Oxoid) were used to identify the types of bacteria.
The antimicrobial susceptibility of 15 of the 40 isolates examined showed that the strains were sensitive to tetracycline, ampicillin, furazolidone, erythromycin, and ciprofloxacin but resistant to trimethoprim-sulfamethoxazole, and also to the vibriostatic compound 0/129.
The antimicrobial resistance was determined, by the agar diffusion disk method for twelve antimicrobials and for the vibriostatic agent, for members of the genus Vibrio, isolated from wild silver mullets (Mugil curema) and from feral and cultured penaeid shrimp (Litopenaeus schmitti, L.
were isolated from stool samples by standard culture procedures, which included introduction onto xylose lysine desoxycholate agar plate and thiosulphate citrate bile sucrose plate, and subsequent screening by triple iron sugar slant (acid butt with no [H.sub.2]S), positive oxidase, negative urease, fermentation of mannitol but not dulcitol and inositol, resistance to vibriostatic agent 0/129, and ability to grow at 0% NaCl.
Its antibiotype showed resistance to trimethoprim-sulfamethoxazole, sulfonamides, trimethoprim, chloramphenicol, streptomycin, and vibriostatic agent O129 (a molecule naturally active against V.
striatum by using colonial morphology, carbohydrate assimilation tests, and sensitivity to amoxycillin and the vibriostatic compound O/129, in conjunction with the API Coryne and API 20NE systems (17).