All isolates were sensitive to O/129 vibriostatic
The biochemical tests were done using commercially available API20E (Biomerieux[C], France) in combination with oxidase, catalase and vibriostatic
Sensitivity was demonstrated with the vibriostatic gent, 0/129 (150 [micro]g/disc and 10 [micro]g/disc).
Sensitivity was demonstrated with the vibriostatic agent 0/129 (150 [micro]g/disc and 10 [micro]g/disc).
Furthermore, Aeromonas's resistance to vibriostatic
compound O/129 (150 pg) and the variable presence of ornithine decarboxylase activities differentiate the genus from the oxidase-positive Enterobacteriaceae-like organisms, Plesiomonas, and Vibrio.
Standard count agar (tSa, Oxoid, UK) for THB and thiosulphatecitrate-bile sucrose (TCBS) agar (Oxoid) as well as sensitivity to the vibriostatic
agent (0/129) (Oxoid) were used to identify the types of bacteria.
The antimicrobial susceptibility of 15 of the 40 isolates examined showed that the strains were sensitive to tetracycline, ampicillin, furazolidone, erythromycin, and ciprofloxacin but resistant to trimethoprim-sulfamethoxazole, and also to the vibriostatic
The taxonomic importance of the vibriostatic
agent O/129 lies in its ability to differentiate species of the genus Vibrio from other gram-negative oxidase-positive bacilli, Aeromonas, in particular.
The antimicrobial resistance was determined, by the agar diffusion disk method for twelve antimicrobials and for the vibriostatic
agent, for members of the genus Vibrio, isolated from wild silver mullets (Mugil curema) and from feral and cultured penaeid shrimp (Litopenaeus schmitti, L.
2]S), positive oxidase, negative urease, fermentation of mannitol but not dulcitol and inositol, resistance to vibriostatic
agent 0/129, and ability to grow at 0% NaCl.
Its antibiotype showed resistance to trimethoprim-sulfamethoxazole, sulfonamides, trimethoprim, chloramphenicol, streptomycin, and vibriostatic
agent O129 (a molecule naturally active against V.
striatum by using colonial morphology, carbohydrate assimilation tests, and sensitivity to amoxycillin and the vibriostatic
compound O/129, in conjunction with the API Coryne and API 20NE systems (17).