Use of cuttings has been a suitable form of vegetative propagation
because of the low cost and success in obtaining uniform crops, with desirable genetic traits (SILVA et al.
However, stem cutting is considered the simplest and most economical method of vegetative propagation
practiced in horticultural industry for mass production within a short time (YOO; KIM, 1996).
Cutting is the most economical method of vegetative propagation
(Davies and Hartman, 1988).
The assets were distributed to all agriculture sectors, and most of the funds are allocated for covering the expenses for vegetative propagation
Although vegetative propagation
is an alternative way for the production of desired clones, propagation through traditional root cutting is not effective due to long procedure and slow growth (Tyagi and Prakash, 2004; Singh et al.
However, it incipient sexual propagation, especially due its low germination rates, its slow growth rate and lack of uniformity (Fossati & Nogueira, 2009) indicate the need for improving vegetative propagation
by cuttings: In March 20th 2014, eight mature shrubs, located in glens consisting of oak forest vegetation, were selected for the harvesting of 96 cuttings from fourteen to eighteen young branches with straight growth.
It was concluded that Naphthalene acetic acid was a better choice for enhanced rooting and vegetative propagation
of Stevia rebaudiana through stem cuttings.
Moreover, the nodes of the stem branches, when stimulated by fragmentation, also thicken and form adventitious roots, thereby increasing the chances for vegetative propagation
(Soares et al.
Up to present air layering has been the only reliable method of vegetative propagation
of guava but this method does not allow production of large number of plants from a limited source of plant mate- rial.
The in vitro culture has been considered an almost indispensable tool to accelerate conventional breeding techniques, and is an alternative to traditional vegetative propagation
and aims to achieve large scale and in a short period of time, free of pathogens and plants identical to the original, in other words, to realize with aseptically perform a plant cloning, which is defined as an asexual propagation of cells or organisms, to obtain new individuals, maintaining the genotype identical to the common ancestor (Torres et al.
In vitro vegetative propagation
has a number of advantages over the sexual one in a large scale reforestation program [1,27,8].