Subsequent hemoglobin electrophoresis cascade revealed a 48.9 percent share of Hb A and a 48.3 percent share of the variant hemoglobin
, which was identified as Hb Sherwood Forest by molecular bidirectional sequence analysis to test for the presence of a mutation in all coding regions and noncoding portions of the beta hemoglobin gene.
Any variant hemoglobin
that is present will be detected as glycated products." (16)
Erythrocytes with variant hemoglobin
S (HbS) undergo sickle-cell process, physiologically caused by low oxygen tension, acidosis and dehydration.
Samples were then run on cellulose acetate electrophoresis at pH 8.9 for pattern analysis and HbA2, HbF, and other hemoglobin variants were quantified by high-performance liquid chromatography using the variant hemoglobin
testing system (Bio-Rad Laboratories, Hercules, CA, USA).
We used the Variant Hemoglobin
Testing System (Variant II Beta Thalassemia Short Program, Bio-Rad Laboratories Inc., Hercules, CA, USA) under the experimental conditions specified by the manufacturer .
Diagnosis of thalassemia was determined after analysis using HPLC (Variant Hemoglobin
Testing System, Bio-Rad, Hercules, CA) for Hb typing and multiplex Gap-PCR as described previously [20, 21].
The FDA cautioned that the test should not be used to diagnose pregnant women, or to monitor diabetes in-patients with severe comorbidities, such as hepatic or renal disease, or with the hemoglobin variant hemoglobin
This test should not be used to diagnose or monitor diabetes in patients with the hemoglobin variant hemoglobin
(6) In a study of 5,136 HbA1c tests performed by one reference laboratory, 1,059 were shown to have a variant hemoglobin
when tested by HPLC.
A new information campaign of the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), part of the National Institutes of Health, highlights the importance of using accurate methods to test hemoglobin A1C in people with diabetes who have sickle cell trait or other inherited forms of variant hemoglobin
Smith CM, Hedlund B, Cich JA, Tukey DP, Olson M, Steinberg MH, Adams JG (1983) Hemoglobin North Shore Caracas: A variant hemoglobin
associated with the phenotype of beta thalassaemia.
phenotypes may account for differential erythropoiesis-stimulating agent dosing in African-American hemodialysis patients.