In problematic cases, (3) performance elastic fiber stain, such as elastic Van Gieson stain, would be useful because the stain demonstrates a more sparse, fragmented, and disorganized elastic fiber distribution in the areas of fibrosis in UIP4 compared with more marked and established elastic fiber deposition in relation to the alveolar walls in PPFE, which is described as containing at least twice as much elastin compared with UIP.
Low-power view showing dense pleural fibrosis associated with subjacent intra-alveolar fibrosis and accompanying septal elastosis, the latter being better seen on elastic Van Gieson stain.
Note the dense pleural fibrosis and the lack of elastosis in the lung parenchyma away from the subpleural region (elastic Van Gieson stain, original magnification x20).
After one month of the primary operation, some brain tissue was taken out with a small elliptical incision under local anaesthesia for serial histological evaluation by Haematoxylin and Eosin stain, (10) PAS stain, (11) Van Gieson stain
, (12) Gomori stain, (13) Giemsa stain, (14) etc.