Masson's trichrome and Van Gieson
's stains reveal the abundance of blood vessels around the duct (Fig.
Elastic van Gieson
staining highlights a layered accumulation of elastic fibers (Figure 2, F).
At 90% confluency, the hFOB 1.19 cells were stained using the Elastica van Gieson
Kit (Merck Millipore, Product Code: 115974) to detect the presence of collagen and Alizarin Red staining (Sigma--Aldrich, Product Code: A5533) to detect the presence of calcium.
Reagent Kit (Beijing Yili Chemical Co., Ltd., China) was used for Van Gieson
stain., To determine the degree of collagen fiber accumulation, we randomly selected twenty fields in three individual sections and calculated the ratios of the areas of Van Gieson-stained interstitial fibrosis to the total ventricular area using software Image-Pro Plus 6.0 (Media Cybernetics, Maryland, USA).,
These sections were subsequently stained with Miller's Elastica van Gieson
stain (EvG) and hematoxylin and eosin (HE).
Paraffin-embedded sections (5 [micro]m) were stained with hematoxylin and eosin (H&E) for assessment of eosinophil infiltration, with periodic acid-Schiff (PAS) for assessment of mucus secretion, and with Van Gieson
(VG) for assessment of collagen deposition.
After polishing to a thickness of 50 [micro]m, these sections were stained in 1.2% trinitrophenol solution as well as 1% acid fuchsin solution (Van Gieson
(c) Elastica van Gieson
(EVG) staining (x40) of the lung showing destruction of the arterial medium.
Then, the slides were rinsed in tap water, treated with 1% acetic acid for 5 min, and counterstained with Van Gieson
1.02439, Merck, Darmstadt, Germany) and Van Gieson
stains to evaluate Ishak modification of hepatitis activity index.
These globules show a serrated edge, highlighted by van Gieson