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Next day two-cell embryos were collected from donor with vaginal plug through flushing of oviduct by M2 medium.
Materials and Methods: Thirty mice, 6 - 8 weeks old weighing 30 - 35 gm were housed together for mating and pregnancy was confirmed by vaginal plug. The fetuses were delivered and dissected on the 19th day and the fetuses were delivered, microscopic slides of the hair follicles small pieces of skin were removed and processed for to be examined under light microscope.
Presence of vaginal plug was taken as day 0 of gestation.