Histopathological findings in cochlear specimens stained with H&E Normal Saline Mercurochrome Gentamicin n:7 n:7 n:7 Hydropic and vacuolar
degeneration in organ of corti 0 3 3 0 3 3 1 2 3 0 2 3 0 3 3 0 2 3 0 3 2 Degeneration in spiral ganglion cells 0 3 3 1 3 2 1 2 3 0 2 3 0 3 3 1 2 2 0 3 2 Hydropic and vacuolar
degeneration in stria vascularis 0 3 2 0 2 3 1 2 3 0 2 2 0 3 3 0 2 3 0 3 2 Organ of corti (Hydropic and vacuolar
degeneration, loss of inner and outer hair cells) Spiral Ganglion (Cytoplasmic and nuclear condensation, loss of nucleus and neurons) Stria Vascularis (Hydropic and vacuolar
degeneration) 0: No degeneration 1: Mild degeneration 2: Moderate degeneration 3: Severe degeneration
The relative area of tubules with cytoplasmic vacuolar
degeneration in the model group was significantly larger than that in the control group at the end of the 12th week (39.0% [+ or -] 9.5% vs.
LP pigmentosus showed saw-toothed rete ridges/ irregular acanthosis, vacuolar
degeneration of the basal layer, band-like infiltrate, melanophages in upper dermis, Civatte bodies, pigment incontinence, chronic inflammatory infiltrate and perivascular infiltrate.
Additionally, 300 mg developed focal necrosis, and hydropic and vacuolar
degeneration of fetal liver tissues.
Also, improvement of the hepatocytes was noticed where the vacuolar
degeneration and inflammatory cells infiltration were decreased that was in consistence with Amin et al.
It has been proposed that certain stress conditions such as low temperature could cause injury to vacuolar
membranes in plant cells [32, 33].
Classic LOPED histopathology shows a vacuolar
myopathy with abnormal glycogen accumulation, which may affect preferentially either type 1 or type 2 fibers, and increased staining for acid phosphatase activity.
The nucleus was circular with a high degree of vacuolar
enlargement and pleomorphism.
Profiles show similarities in vacuolar
pathology intensity and distribution in wild-type mouse brains.
Histopathologic examination of the skin biopsy revealed atrophic epidermis and vacuolar
degeneration of the basal layer (Figures 2a, b).
Photomicrographs of hematoxylin and eosin stained histological section of (a) normal liver, (b) C[Cl.sub.4]-treated animals showing variable degrees of vacuolar
degeneration, (c) C[Cl.sub.4]-treated animals showing widened sinusoids, active proliferated Von kupffer cells, (d) C[Cl.sub.4]-treated animals showing necrotic changes in the form of severe destruction of hepatocytes or karyolysis, (e) C[Cl.sub.4]-treated animals showing leucocytic infiltration in the portal area and hepatic parenchyma, (f) C[Cl.sub.4]-kefir treated animal showing much less damages in the hepatic parenchyma, more or less normal hepatocytes, and (g) C[Cl.sub.4]-kefir treated animal showing very mild vacuolar
degeneration with no evidence of necrosis, mild congestion and no inflammatory changes
Additionally, in the animal that was dosed 20g [kg.sup.-1] and died at 29.75 hours post treatment, hydropic vacuolar
degeneration and necrosis of epithelial cells of the kidney were observed (Figure 2B).