Uric acid can be utilized as a nutrient by termites and cockroaches following digestion by uricolytic bacteria harbored in the hindgut or fat body.
The uricolytic pathway is the main route for uric acid production in insects, starting with the digestion of proteins (Nation 2002).
One theory holds that uric acid in fat body is transported via the Malpighian tubules to the hindgut, where it undergoes anaerobic digestion by uricolytic bacteria (Breznak 2000).
A second theory submits termites permanently excrete uric acid to fat body, with nitrogen for amino acid synthesis predominantly provided by nitrogen-fixing spirochetes in the hindgut rather than uricolytic bacteria (Slaytor and Chappell 1994).
This was attributed to digestion of urates by uricolytic
bacteria in the hindgut (Potrikus & Breznak 1980b, 1980c).
Rasburicase, a recombinant uricolytic
agent, has a new indication for the initial management of plasma uric acid levels in adults with leukemia, lymphoma, and solid tumor malignancies "who are receiving [anticancer] therapy expected to result in tumor lysis and subsequent elevation of plasma uric acid."
To investigate its in vitro uricolytic activity, rasburicase (concentration: 1.5 mg/L) was added to heparinized blood samples from healthy volunteers (n = 4).
(1, 2) who demonstrated that the in vitro uricolytic activity of rasburicase is minimized in samples maintained at 4 [degrees]C.
Many cockroaches harbor uricolytic
bacteria in their fat body that digest uric acid from adjacent storage sites (Cochran, 1985).
Rasburicase is a potent uricolytic agent that catalyzes the enzymatic oxidation of uric acid to allantoin, a water soluble metabolite that is readily excreted by the kidney (1).
This prompted us to evaluate the magnitude of in vitro uricolytic activity of Rasburicase, and we undertook two experiments.
This finding demonstrates that the in vitro uricolytic activity of Rasburicase is minimized in a sample maintained at 4 [degrees]C as reported previously (1, 3).