Effect of postsymptom application of fungicides on urediniospore
production by Puccinia triticina on wheat and P hemerocallidis on daylily.
DNA was extracted from urediniospores
collected from field infected and inoculated 'Alamo' plants and primers Rust1 (2) and PuccF (5'-CAATGGATCTCTAGGCTCTC-3', B.
The numbers of urediniospores
of each strain inoculated onto the leaves in each pot were estimated by observing urediniospore
deposition on clear plastic strips in the pots (method described in Inoculation).
subglobose or ovoid, yellowish brown, 12-18 x 16-26 um; wall densely echinulate, germ pores 2-4,scattered.
double dagger]) Units are on a scale of 0 = no uredinia, 1 = chlorotic flecks, 2 = small uredinia surrounded by necrosis with limited urediniospore
production, 3 = medium size uredinia with moderate sporulation, and 4 = large uredinia and abundant sporulation.
tritici pathotype QCCJ at the early jointing stage by injecting an aqueous suspension of urediniospores
into the hollow culm of plants chosen at random at [appproximately equals] 0.
UREDINIOSPORES globose to ellipsoid, light brown, wall chestnut brown, 20-32.
on a water-agar medium supplemented with 1 mL of water containing 1% Tween 20 (negative control) allowed 74% spore germination.
Uredinia are observed initially in the dark dots that progressively acquire a light brown to red brown color and open in a minute pore releasing the colorless urediniospores
, which become tan and accumulate around the pores (Balardin, 2002; Yorinori et al.
8 mm wide, erumpent, powdery, without peridium, orange-yellowish fading to pale yellow; urediniospores
25-32 x 16-24 [micro]m, broadly ellipsoid to subglobose or angular, catenulate, coarsely verrucose, wall 1.
Two types are found on daylilies in the United States and Costa Rica: urediniospores
Deposition gradients of urediniospores
of Puccinia recondita near a source.