When UF6 comes into contact with water, such as water vapour in the air, it forms corrosive hydrogen fluoride (HF) and a uranium fluoride compound called uranyl
Because of their high affinity for phosphate, carboxyl, and hydroxyl groups, uranyl
compounds readily combine with proteins and nucleotides to form stable complexes (Moss 1985).
Thin sections were cut with a diamond knife on a ultramicrotome and mounted on uncoated 200-mesh gold grids and stained with uranyl
acetate and lead citrate (Hayat, 1972).
The ultrathin sections were stained with uranyl
acetate and lead citrate and observed under an electron microscope (JEOL1400 Tokyo Japan) and electron micrographs were captured at the same time.
Infected Vero cell monolayers were fixed in a modified Karnovsky solution, stained with uranyl
acetate and lead citrate, and examined in a transmission electron microscope according to standard procedures.
1] sodium cacodylate, and stained en bloc for 1 h in 3% uranyl
acetate in 0.
The thin sections were made and colected in 200 mesh, counterstained with uranyl
acetate (Watson, 1958) and lead citrate (Reynolds, 1963) and examined in a JEOL-1010, transmission electron microscope at 80 kV.
acetate (Fluka Chemie GmbH, Sigma-Aldrich, Buchs, Switzerland).
Ultrathin sections were stained with uranyl
acetate and lead citrate and examined with a transmission electron microscope (H-600, Hitachi, Tokyo, Japan).
TEM sections were placed on 200-mesh copper grids, stained with uranyl
acetate (Watson, 1958) and lead citrate (Reynolds, 1963) for 45 min and 15 min, respectively, and observed and photographed by using Kodak SO-163 Electron Image film (Kodak Co.
Under oxidizing conditions, U (VI) mainly exist in the form of complexed, adsorbed, or precipitated uranyl
Thereafter, using a suitable microtome for ultra-thin sectioning, 60 - 80 nm thick sections of the fixed tissue were cut and double-stained with 3% uranyl
acetate, followed by lead citrate, and then examined using the TEM (JEM2100HC, JEOL Ltd, Tokyo, Japan).