Initially,
uracil (2.0 g) was dissolved in distilled water (8.0 mL).
Peak 1:
uracil; peak 2: uridine; peak 3: adenine; peak 4: guanosine; and peak 5: adenosine.
Distances of interactions in [Angstrom]ngstroms are as follows: 3.3 from 3'-acyl carbonyl O to [G143.sub.0.3] N 3.2 from
uracil O4 to [N198.sub.01] N[delta], 3.0 from
uracil O4 to [R205.sub.0.1] N[eta]2 2.7 from
uracil N3 to [N198.sub.0.1] O[delta], 2.9 from water to [H99.sub.0.3] N[delta], 2.8 from same water to [beta]-hydroxyl, 2.8 from [beta]-hydroxyl to H12203 N[epsilon], 3.0 from [beta]-hydroxyl to [Q73.sub.0.3] N[epsilon], 3.3 from glucosamine 3'O to [H125.sub.0.3], 2.8 from [H125.sub.0.3] NS to [D126.sub.0.3], 2.9 from glucosamine 6'-hydroxyl to [H144.sub.0.3] N[epsilon], 3.0 from the glucosamine ring O to [K76.sub.0.3] N[zeta], 3.0 from 2'-acetyl carbonyl to [L75.sub.0.3] N, and 2.6 from [alpha]-phosphate to [Q161.sub.0.3] N[epsilon].
Our previous studies identified that
uracil lesions in the DNA of formalin-fixed tissues are the major source of C>T (and G>A) sequence artifacts (13, 14).
sinensis based on biosynthetic pathway and differential expression analysis, the uridine production reached 5.04 mg/g with an increase of 28.6% when 2 mg/mL ribose was added, while uridine production reached 10.83 mg/g with a rise of 176.3% when 0.3 mg/mL
uracil was added, indicating that the product inhibition for uridine nucleosidase by
uracil was stronger than that by ribose.
Absorbance measurements were made on the TRIS buffer, tryptophan solution, and
uracil solution using the calibrated short-pathlength cuvettes on the Cary transfer spectrophotometer.
The resulting
uracil prototroph transformants exhibit blue colonies and can be selected on uracil-free plates.
TLC of the solid (dissolved in water, eluted with a 70: 40: 10 by volume mixture of ethyl acetate-acetone-water and visualized by UV) showed
uracil as a major component (Shyamaprosad et al, 2007)
Folate mediated one-carbon metabolic pathway is central to DNA metabolism including its synthesis repair and methylation.1 This strategy is utilized therapeutically for hematological and trophoblastic malignancies.23 The one carbon metabolism also involves regulation of epigenetic mechanisms including CpG island and histone methylation and DNA
uracil incorporation hence not surprisingly a number of studies have shown aberrant folate and vitamin B12 metabolism to be associated with cardiovascular risk4 neurodegeneration5 and malignancies of various tissues.6-10 There are only some studies that explore the status of folate and vitamin B12 in acute lymphoid leukemias (ALL).
Moreover the importance of
uracil and its annulated derivatives is well recognized by synthetic [26, 27] as well as biological [28, 29] chemists.
Bromacil is a widely used herbicide in the substituted
uracil family that is commercially used in the United States for annual and perennial brush and weed control in citrus and pineapple crops as well as in nonagricultural settings [1].
The stereoisomeric aminols 11a,b were also converted into the
uracil and thymine nucleosides.
)