microspores gradually increased in volume and the number of vacuoles (Figs.
Although it may be premature to propose that this infection can cause host mortality, one of the crabs (#68; see Table 1) that failed to molt had large numbers of uninucleate
and plasmodial forms of mikrocytid parasites in the hemolymph with evidence of necrosis in the epithelial cells of the antennal gland.
Once polyploid uninucleate
cells are established, the chalazal cellular tissue becomes mitotically and cytokinetically active and enters a period of extensive growth.
tapetal cells were commonly observed.
containing phagocytes varied from 1-5, with 2-3 oocysts being the most frequent condition, with a single uninucleate
sporozoite in each oocyst.
Microspore isolation: Flower buds containing microspores at the late uninucleate
stage of development were used.
The mid to late uninucleate
microspores were the most responsive to chemical induction of androgenesis.
For most species the only period of microspore sensitivity to inductive pretreatments revolves around the transition from the uninucleate
, vacuolate microspore to early, bicellular pollen, i.e.
There were 1-2 oocytes found per host cell (phagocyte), with the oocyst containing a single uninucleate
microspore stage is uniformly recognized as the most suitable stage of pollen development for initiating androgenesis with greatest chances of success.