The
uninucleate microspores gradually increased in volume and the number of vacuoles (Figs.
Although it may be premature to propose that this infection can cause host mortality, one of the crabs (#68; see Table 1) that failed to molt had large numbers of
uninucleate and plasmodial forms of mikrocytid parasites in the hemolymph with evidence of necrosis in the epithelial cells of the antennal gland.
Once polyploid
uninucleate cells are established, the chalazal cellular tissue becomes mitotically and cytokinetically active and enters a period of extensive growth.
The
uninucleate tapetal cells were commonly observed.
containing phagocytes varied from 1-5, with 2-3 oocysts being the most frequent condition, with a single
uninucleate sporozoite in each oocyst.
Microspore isolation: Flower buds containing microspores at the late
uninucleate stage of development were used.
The mid to late
uninucleate microspores were the most responsive to chemical induction of androgenesis.
For most species the only period of microspore sensitivity to inductive pretreatments revolves around the transition from the
uninucleate, vacuolate microspore to early, bicellular pollen, i.e.
There were 1-2 oocytes found per host cell (phagocyte), with the oocyst containing a single
uninucleate sporozoite.
The
uninucleate microspore stage is uniformly recognized as the most suitable stage of pollen development for initiating androgenesis with greatest chances of success.