The semithin plastic sections (1 im) of gBMSC-seeded silk fiber were cut using an ultramicrotome
with glass knives, stained with 1% Toluidine Blue, viewed under the light microscope (Nikon) and photographed.
As an artificial substrate, the threads were easily sectioned with a diamond-knife mounted on an ultramicrotome
Semithin (1 to 2 pm) sections were cut with glass knives on an ultramicrotome
and stained with toluidine blue and basic fuchsin for light microscopic examination with bright-field and differential interference contrast optics.
Ultrathin sections were cut on a Porter-Blum MT2 ultramicrotome
, stained with uranyl acetate and lead citrate, and examined with a Hitachi H-600 transmission electron microscope.
The nerve segments were washed again three times with the cacodylate buffer and then immersed in 50% ethanol and 3% uranyl acetate for 90 min followed by dehydration through a graded ethanol series, and were left overnight in absolute ethanol--Spurr's resin solution (1:1), followed by Spurr's resin alone for 8 hrs at room temperature and then overnight in a 70[degrees] C oven prior to sectioning by the aid of an ultramicrotome
for light and electron microscope observations.
The ultrathin slices (about 80-90 nm) were prepared by ultramicrotomed diamond knife of the RMC-MT-X ultramicrotome
(Boeckeler Instruments, Inc.
60-90 nm sections were prepared with LKBUM4 ultramicrotome
using a diamond knife and sections were mounted on a copper grid and stained with uranyl acetate and reynolds lead citrate.
Specimen blocks were trimmed then sectioned at 3 [micro]m using a Sorvall MT-2b ultramicrotome
Sections were obtained with a Power Tome XL ultramicrotome
(Boeckeler Instruments, Tucson, AZ, USA).
Transverse, ultrathin sections were obtained using a Leica Ultracut UCT ultramicrotome
with a diamond knife.
Semi-thin and thin sections were cut with an ultramicrotome
Ultrathin sections were cut with an Ultracut S ultramicrotome
(Leica, Wetzlar, Germany), mounted on copper grids, stained with uranyl acetate and lead citrate, and studied in an EM 902 A TEM with electron energy loss spectroscopy (EELS) or Libra 120 TEM with EELS and electron-dispersive X-ray spectroscopy (Carl Zeiss AG).