Ultrathin sections of the fibres (50--70 nm) were cut by an ultramicrotome
(Leica Ultracut UCT) using diamond knife (Diatome, Leica), stained with Uranyl acetate (EM Sciences)--Lead citrate (Ladd Chemicals, USA) and imaged under the microscope (Hitachi H-600).
6 pm ultrathin sections were cut using a glass knife on an LKB V Ultramicrotome
(LKB Company, Bromma, Sweden).
To prepare the polymer samples, slices of 5 lm thickness were first cut by ultramicrotome
(Ultrotome 2128 by LKB), melted between two microscopy cover glasses, and pressed gently to reduce thickness.
These samples were postfixed with 1% osmium tetraoxide, dehydrated with alcohol, included in plastic resin (EPON), cut with an ultramicrotome
and contrasted with uranyl acetate and lead citrate.
Ultrathin sections were cut with a Supernova ultramicrotome
(Reickert Jung, Vienna, Austria), mounted on copper grids, stained with uranyl acetate and lead citrate and then observed and photographed with a Philips CM10 transmission electron microscope (TEM; Philips Scientifics, Eindhoven, The Netherlands).
One section with 60 nm-thickness was cut from each epon block using a ultramicrotome
Thin sections (50-70 nm) were cut by a Leica UCT-125 ultramicrotome
(Leica Microsystems GmbH, Vienna-Austria) and contrasted with uranyl acetate and lead citrate.
Embryonic or juvenile feather filaments were sectioned at 1-3 [micro]m thickness in both cross, oblique and longitudinal planes using an ultramicrotome
5 [micro]m using a histodiamond knife (Diatome, Electron Microscopy Sciences, Inc) on an ultramicrotome
and stained them with toluidine blue.
Leica also introduced a number of specimen preparation products, including a high pressure freezer, automatic freeze substitution systems anda new ultramicrotome
Several instruments were used to prepare samples including a Micrion 2500 Focused Ion Beam (FIB) instrument, a Leica Ultracut UCT ultramicrotome
equipped with a diamond knife, and a Gatan Model 691 Precision Ion Polishing System (PIPS).
Finally, the samples were stored at 37[degrees]C for 48 hours, and 60 nm slices were taken with a LKB Novo ultramicrotome