of Cases Source, y Reported Stained This study 22 22 Amin et al, (1) 1994 18 6 Johansson et al, (7) 1999 20 20 Maranchie et al, (8) 2000 5 3 Oh and Kim, (9) 2000 1 1 Vera et al, (10) 2002 1 1 Vera et al, (10) 2002 2 2 Endothelial Source, y Stains Used Results This study CD34 and D2-40 21/22 with focal LVI Amin et al, (1) 1994 FVIIIR:Ag, UEAI 6/6 cases with focal LVI Johansson et al, (7) 1999 FVIIIR:Ag 15/20 cases with focal LVI Maranchie et al, (8) 2000 CD31 0/3 cases with LVI Oh and Kim, (9) 2000 CD31 1/1 with focal LVI Vera et al, (10) 2002 CD31 and CD34 1/1 with focal LVI Vera et al, (10) 2002 CD34 0/2 with LVI Abbreviations: FVIIIR: Ag, rabbit anti-human factor VIII-related antigen; UEAI, rabbit anti-human Ulex europaeus
agglutinin I lectin.
The culture supernatants when tested for potency, avidity and specificity, gave a potent titre of 1:64-1:128 compared to the lectin Ulex europaeus
titre of 1:8-1:16 (10).
Lectin binding to oyster digestive tissues and inhibitory effect on binding of Norwalk viruslike particles (VLPs) Binding ([micro]g/mL) VLP inhibition ([micro]g/mL) Lectin * ([dagger]) ([double dagger]) DBA 1 None UEA-1 50 None HPA 1 25 * Derived from Dolichos biflorus (DBA), Ulex europaeus
(UEA-1), and Helix pomatia (HPA).
Further immunochemical studies on the combining sites of Lotus tetragonolobus and Ulex europaeus I and II lectins.
a]; HPA, Helix pomatia agglutinin; UEA 1, Ulex europaeus I; GlcNAcT-V, UDP-GlcNAc:N-glycan GlcNAc transferase V; MMP, matrix metalloproteinase; ADAM, a disintegrin and metalloproteinase; ECM, extracellular matrix; EGF, epidermal growth factor; EGFR, EGF receptor; T[beta]R, transforming growth factor [beta] receptor; LC-MS/MS, liquid chromatography-tandem mass spectrometry; PNGase, peptide N-glycosidase; AAL, Aleuria aurantia lectin; LCA, Lens culinaris agglutinin; LEL, Lycopersiconesulentum lectin; MRM, multiple reaction monitoring; SISCAPA, stable isotope standards and capture by antipeptide (or anticarbohydrate) antibodies; MUC1, mucin-1.
The lectin sequences from Maackia amurensis (NCBI Entry AAB39934), Ulex europaeus
(NCBI Entry AF190633), Lens culinaris (NCBI Entry AAY21161), Vicia faba (NCBI Entry CAD27436), Arachis hypogaea (NCBI Entry AY431029) and Canavalia ensiformis (NCBI Entry AF308777) were acquired from the GenBank database.
Because these tumors arise from endothelial cells, they usually express factor VIII-related antigen and/or ulex europaeus agglutinin-binding protein.
All cases were also stained with antibodies for factor VIII-related antigen (Dako), ulex europaeus agglutinin (Dako), and CD34 (BioGenex, San Ramon, Calif) using the same standard technique.
All cases, except one (case 11), were immunoreactive for at least 2 additional vascular markers (factor VIII-related antigen, ulex europaeus agglutinin, and CD34).
The tumor cells in angiosarcomas are generally immu noreactive with the Ulex europaeus
lectin type i (UEA-1),[7-10] vimentin,[8,9,11] and with the endothelial markers factor VIII, CD34, and CD31.
All angiosarcomas and 2 carcinomas were positive for Ulex europaeus