Long term culture of the a549 cancer cell line promotes multilamellar body formation and differentiation towards an alveolar type ii pneumocyte
Areas of type II pneumocyte
proliferation were observed.
The characteristic histopathological pattern of IPF includes type II pneumocyte
hyperplasia and abnormal mesenchymal cell proliferation.
Lamellar bodies are secreted by the type II pneumocyte
and unfold to form tubular myelin and other large aggregates that are absorbed onto the hydrated inner layer of the alveoli.
Histologic analysis showed that lesions were similar to those observed in humans and indicated necrotizing bronchointerstitial pneumonia with type II pneumocyte
hyperplasia, abundant fibrin, and streaming mucus in airways.
In contrast, lungs involved with diffuse alveolar damage exhibit widened alveolar septa, reduced numbers of aerated airspaces, and other histologic changes of injury, including the presence of hyaline membranes lying within airspaces, and type II pneumocyte
hyperplasia (Figure 1, C through E).
Alveolar walls were thickened and showed necrosis of lining epithelium and type II pneumocyte
The renewal strategy of alveolar epithelium is different from that operating in conducting airways, with the type II pneumocyte
exerting both specialized (eg, the production of surfactant proteins) and precursor functions.
Cellular debris, including degenerating alveolar macrophages, sloughed pneumocytes, and cholesterol clefts, is often present, and there may be a regenerative type II pneumocyte
The shape and location of the antigen-positive cells indicated that they were type II pneumocytes
. To confirm this, we used surfactant as a marker of type II pneumocyte
There is evidence that sclerosing hemangioma of the lung shows evidence of type II pneumocyte
In summary, we believe that the uniform and strong positivity for TTF-1 in both pale cells and cuboidal cells supports the notion that this neoplasm shows evidence of type II pneumocyte